You were asked to determine the mode of inhibition exerted by Inhibitor DEDS to a newly discovered enzyme known as BILISTASE. The kinetics data are shown below: [Substrate, uM] Vo with DEDS (µM/min)...

33 .You were asked to determine the mode of inhibition exerted by Inhibitor DEDS to a newly discovered enzyme<br>known as BILISTASE. The kinetics data are shown below:<br>[Substrate, uM]<br>Vo with DEDS (µM/min)<br>Vo without DEDS (µM/min)<br>3.333<br>0.774<br>1.196<br>4.000<br>0.877<br>1.316<br>5.000<br>1.012<br>1.462<br>10.000<br>1.462<br>1.880<br>20.000<br>1.880<br>2.193<br>Using linear regression analysis, determine the values of Vmax and KM of the enzyme in the ABSENCE of<br>inhibitor: (Express your answer in 3 decimal places, do NOT include the units)<br>V max<br>%D<br>KM =<br>

Extracted text: You were asked to determine the mode of inhibition exerted by Inhibitor DEDS to a newly discovered enzyme known as BILISTASE. The kinetics data are shown below: [Substrate, uM] Vo with DEDS (µM/min) Vo without DEDS (µM/min) 3.333 0.774 1.196 4.000 0.877 1.316 5.000 1.012 1.462 10.000 1.462 1.880 20.000 1.880 2.193 Using linear regression analysis, determine the values of Vmax and KM of the enzyme in the ABSENCE of inhibitor: (Express your answer in 3 decimal places, do NOT include the units) V max %D KM =

Jun 11, 2022
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