NHMRC additional levels of evidence and grades for recommendations STAGE 2 CONSULTATION NHMRC additional levels of evidence and grades for recommendations for developers of guidelines STAGE 2...

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Answered Same DaySep 22, 2021DTN3NDSLa Trobe University

Answer To: NHMRC additional levels of evidence and grades for recommendations STAGE 2 CONSULTATION NHMRC...

Sunabh answered on Sep 24 2021
163 Votes
Running Head: HEALTHCARE        1
HEALTHCARE        6
Student Number: ____________________
Subject Code: ____________________
Name: ____________________
Title of Assessment: Microbiome Assignment
Chosen Article: Watson, H., M
itra, S., Croden, F. C, Taylor, M., Wood, H. M., Perry, S. L., ... & Dye, L. (2018). A randomised trial of the effect of omega-3 polyunsaturated fatty acid supplements on the human intestinal microbiota. Gut, 67(11), 1974-1983
Word Count: 760 words
Submission Date: ____________________
1. In your own words, what was the purpose of the study (why did the authors conduct it?)
The purpose of this study by Watson et al. (2018) was to identify the effect of omega-3 polyunsaturated fatty acids (PUFAs) on the faecal micro-biome within middle aged and healthy volunteers because previous studies proposed anticolorectal cancer (CRC) activities of omega-3 polyunsaturated fatty acids (PUFAs). Therefore, the major aim was to reflect a relationship between intestinal micro biome and systemic omega-3 PUFA exposure.
2. What is the study design? (What type of study is reported in the paper?) 
The study design of this research was randomised, open-label, cross-over trial for next 8 weeks where the treatment was done with 4 g mixed eicosapentaenoic acid/ docosahexaenoic acid. Faecal samples were collected at 5 times point during the study for micro-biome analysis.
3. What were the exposures examined in this study? How were they defined and measured?
The exposures were eicosapentaenoic acid/docosahexaenoic acid given in two formulations as soft-gel capsules and Smartfish drinks, which were then separated by a ‘washout’ period of 12-weeks. Faecal samples were collected for microbiome activity analysis through 16s rRNA PCR as well as Illumina MiSeq sequencing.
4. What was the main outcome examined in this study? How was it defined and measured? 
Major outcome examined from this study reflected no significant relationship between intestinal micro-biome and systemic omega-3 PUFA exposure. Illumina MiSeq sequencing and 16S ribosomal RNA PCR were the techniques used to analyse faecal samples collected at 5-time points. Results also reflected that both...
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