You have run a gel with 5 uL of amplified DNA in addition to 1 uL of 6X loading dye. Comparing the brightness of this band to your marker, you estimated that your amplified DNA is approximately 62 ng....

Extracted text: You have run a gel with 5 uL of amplified DNA in addition to 1 uL of 6X loading dye. Comparing the brightness of this band to your marker, you estimated that your amplified DNA is approximately 62 ng. What was the concentration of your amplified DNA that was loaded? You do not need to account for the volume of loading dye in your calculations.