Working for the EPA, you have isolated several E. coli strains contaminating a pond near a sewage treatment facility. Using an antibody-based diagnostic kit, you have obtained the results shown below (Figure 3) on your dot blot (similar to a Western blot, but the protein sample is directly applied as a spot to the membrane without sodium dodecyl sulfatepolyacrylamide gel electrophoresis being run first). Based on these results, predict the identity of each of the unknown isolates. Be sure to briefly state your rationale. CNF, cytotoxic necrotizing factor.
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