Determine Km, Vmax, and kcat (in 1/s) for VF-6-2 efflux out of vesicles made from DV membranes with wild type PfCRT. What does the mutation in PfCRT appear to do to transport of VF-6? Wild type PFCRT...

Determine Km, Vmax, and kcat (in 1/s) for VF-6-2 efflux out of vesicles made from DV membranes with wild type PfCRT. What does the mutation in PfCRT appear to do to transport of VF-6?

Wild type PFCRT activity<br>1.2<br>1<br>0.8<br>y = 10.64x + 0.0569<br>R2 = 0.9977<br>0.6<br>0.4<br>0.2<br>0.02<br>0.04<br>0.06<br>0.08<br>0.1<br>0.12<br>1/[VF-6] (1/uM)<br>1/VF-6 efflux (h/pmol)<br>

Extracted text: Wild type PFCRT activity 1.2 1 0.8 y = 10.64x + 0.0569 R2 = 0.9977 0.6 0.4 0.2 0.02 0.04 0.06 0.08 0.1 0.12 1/[VF-6] (1/uM) 1/VF-6 efflux (h/pmol)

Jun 11, 2022

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Determine Km, Vmax, and kcat (in 1/s) for VF-6-2 efflux out of vesicles made from DV membranes with wild type PfCRT. What does the mutation in PfCRT appear to do to transport of VF-6? Wild type PFCRT...

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