Why do you measure the absorbance at 595 nm when do in the Bradford assay? That is the wavelength of maximum aborbance for the Bradford reagent in its blue form. That is the wavelength of maximum...

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Extracted text: Why do you measure the absorbance at 595 nm when do in the Bradford assay? That is the wavelength of maximum aborbance for the Bradford reagent in its blue form. That is the wavelength of maximum aborbance for the Bradford reagent in its red form. That is the wavelength of maximum sensitivity for the spectrophotometer. O It doesn't really matter. Any wavelength would work fine.