What is the purpose of washing a western blot membrane with TBST after the primary antibody binding and before substrate addition? To remove excess antibody that is not bound to the protein of...

Extracted text: What is the purpose of washing a western blot membrane with TBST after the primary antibody binding and before substrate addition? To remove excess antibody that is not bound to the protein of interest O To remove the protein of interest from non-specific binding pockets of the membrane. There is no purpose, this is not an important step and may be skipped. To remove the HRP from the primary antibody, allowing for greater accuracy of signal presence.