Virtual Microscope Lab
Objectives:
oLabel the parts of a compound light microscope
oDescribe the functions of the parts of a compound light microscope
oPractice using a microscope to see human cells
oIdentify different tissue types and describe their function
oRelate structure to function
Materials:
·Computer with internet access
·Pencil and paper
·Colored pencils if desired
Procedure:
Go to the website:http://www.ncbionetwork.org/iet/microscope/?fbclid=IwAR0-g53Rb52QQP3SZ3edp-JBP8qWADJfSlBd6mLxnGQMBIFcniCQpaaa5SU
1.Click on the Guide and Learn sections go over all the information and learn the parts.
2.Following these steps from the Introduction Guide
3.Click on the Explore link (bottom of the home page).
4.Click on the question mark on the slide box.
5.In the Slide Catalog, click on the Sample Slides.
6.Click on the Letter E slide. It will automatically be placed on the stage of the microscope.
7.When the Microscope View window opens, make sure that the 4X circle is highlighted in blue. NOTE: Always begin examining slides with the lowest power objective.
8.Use the slider under Coarse Focus to find the E. NOTE: The coarse adjustment knob should only be used when you are viewing a specimen with the 4X or 10X objective lenses.
9.Then use the slider under Fine Focus to make the image “crisp and clear.”
10.You can click on the E in the viewing window to move the image and visualize different parts. Sketch your view of the letter E at 4X.
11.Next click on the 10X circle. The nosepiece on the microscope will rotate automatically.
12.Repeat steps 6 – 8 to see part of the E. Sketch your view of the letter E at 10X.
13.Click on the 40X circle and repeat steps 7 & 8. You may need to use the slider under Light Adjustment for better visualization. Sketch your view of the letter E at 40X.
14.Click on the Explore link (bottom of the home page).
15.Click on the question mark on the slide box.
16.In the Slide Catalog, click on the Sample Slides.
17.Choose Human Slides
18.Find the following slides, focus them on 40x and make drawings of each at that power.
oEpithelial (simple squamous, and one other)
oBlood
oBone
oAdipose
oTendon
·Label each drawing with the cell membrane and nucleus.
·Label the red and white blood cells.
Find microscope pictures of the following human tissues and make a drawing of each and label each drawing with the cell membrane and nucleus.
o3 types of muscle
oNeurons
For the report
19.Label the parts of the attached microscope diagram and describe their function.
20.Why is the image inverted and backwards in a traditional compound light microscope?
21.Why do you need to adjust the diaphragm when you change objectives?
22.What would happen if you used the course adjustment on high power?
23.Write out the steps to view and focus a specimen starting at 4x and moving to 100x.
Answer the follow-up questions:
1)How is the microscope useful in modern biology?
2)How is knowledge about the structure of a tissue useful?