UTSC Journal of Plant Stress BIO A02 WINTER - 2022; 1(1): 1-6 Insert Principal Author’s Name/Student Numbe Paper title (The title should be descriptive, specific, and concise-Do not use “Formal Lab...

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Answer To: UTSC Journal of Plant Stress BIO A02 WINTER - 2022; 1(1): 1-6 Insert Principal Author’s Name/Student...

Preeti answered on Mar 15 2022
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UTSC Journal of Plant Stress
BIO A02 WINTER - 2022; 1(1): 1-6
Insert Principal Author’s Name/Student Number
                                    
Effect of salt concentration on the morphology of Arabidopsis thaliana
Saeedeh 1
1Dept. of Biological Sciences, University of Toronto Scarborough, Toronto, Canada
UTSC BIOA02 Lab PRAXX
PRAXX TA:
Abstract: Soil salinisation occurred by both naturally or due to anthropogenic malpractices affects the vegetation adversely. It may affect the water and nutrition uptake of the plant which can hamper the n
atural processes of the plants. In this study we selected four mutant strains of Arabidopsis thaliana because of its small life cycle; sos1-1, abi3-1,Col-0 and Ler-0. The effect of the salinity upon the morphologic characters such as height and weight of these mutant strains were studied. After 4 weeks, the treated group of plants were watered with saline water instead of the tap water. It was found that the saline conditions hindered the height and weight all of the mutant strains when compared to the control group. However when the treated group of different mutant strains were compared it was found that Col-0 and abi3-1 was more adapted to the saline conditions than sos1-1 and Ler-0 respectively.
Keywords: Arabidopsis thaliana, Salinity, Mutants, Stress, Response
Introduction
Plant growth , morphology, weight etc. are generally measured to see the effect of any environmental stress such as temperature, salt concentration, drought etc (Harb et al., 2010). Soil salinisation occurred by both naturally or due to anthropogenic malpractices affects the vegetation adversely. It may affect the water and nutrition uptake of the plant which can hamper the natural processes of the plants. The salinity in soil affects the growth of plants and also agricultural production (Shrivastava & Kumar, 2015). The very first thing that is affected by salinity is the roots of the plants. There is an accumulation of phytotoxic ions which causes osmotic shock in plant roots (Hernández, 2019). It further affects the nitrogen uptake of plants, its growth and the reproduction in plants.
In our study, we have used Arabidopsis thaliana as our model organism to study the effect of salt stress response on plant height and above ground fresh weight. A. thaliana was the first plant whose genome has been completely sequenced. It belongs to the family Brassicaceae. It is a small plant with short life cycle and because of these characteristics it is a well suited plant to study the response of plants under various environmental stresses such as high salt concentration, temperature, drought-response, flood-response, heavy metal ions etc. We selected four mutant strains of A.thaliana; Col-0, sos1-1, aba3-1 and Ler-0. Col-0 is a laboratory strain whose genome has been completely sequenced and is used as a reference for comparison with the chs1-2 and sos1-1 mutant. Col-0 has relatively low levels of seed dormancy. By exposing Col-0 with fast neutrons, sos1-1 was generated. In Landsberg erecta (Ler-0), mutation was induced using X-ray in ERECTA gene. In aba3-1 strain, the Ler-0 was mutated using a chemical mutagen EMS, which increases the resistance towards Abscisic Acid (ABA) in plants.
In this study, 27 samples for both control and drought plants were selected for each strain and the height and weight of the plants were measured after 7 weeks. The experimental plants, which were watered with saline water, were grown with control group of plants, which were watered with tap water, for 7 weeks and their height and weight was measured and compared with control group of plants. We concluded that the saline conditions affected the height and weight of all of the mutant strains. In the presence of saline conditions, the height and above ground fresh weight of the experimental and treated groups was lower than the control groups. It was also found that Col-0 has better adaptability towards the saline condition than sos1-1. Similarly, in the case of Ler-0 and abi3-1, abi3-1 is more adapted in saline conditions than ler-0.
Materials and Methods
Collection and Sterilization of seeds
For the collection of seeds the TA collected the seeds from the source lab in two 1.5ml centrifuge tubes. One tube was taken for control plant groups (which will be grown normally) and another for experimental or treated plant groups (to which saline conditions will be provided). For sterilization, the seeds were washed twice with 0.5 ml of 30% bleach and incubated for 3 minutes. Then, the bleach was removed, and the seeds were washed with 200μl of distilled water twice. This step of washing with bleach and then, with distilled water is repeated twice.
Transfer of plant seeds to MS Media plates
After sterilizing the seeds with hypo or bleach, 100 μl of cooled 0.1% agarose was added to seeds and mixed gently. 1ml Pipette tips were used to dot the agarose-seed solution on MS agar plates. The plates were wrapped in aluminium foil to keep them away from light and kept in dark at 4oC for 3 days. After three days, the plates were transferred to growth chamber with day length of 16 hours and night period of 8 hours at 22oC. The average relative humidity (RH) in the growth chamber was 60%. For the next few days, the growth was observed. After that, the transfer...
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