Using the CCD detectors and system magnifications in Exercise 12.8, assume that a z-stack of 100 images is acquired with steps of 50 nm. Calculate in image space the height of the full z-stack and the...


Using the CCD detectors and system magnifications in Exercise 12.8, assume that a z-stack of 100 images is acquired with steps of 50 nm. Calculate in image space the height of the full z-stack and the height of a single 50-nm step. (See Section 13.3.2 for a treatment of magnification in the z dimension, and state any assumptions that you make in your calculations.)



May 03, 2022
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