Using a diagram similar to those provided, describe how serial dilutions and the Standard Plate Count (SPC) allow a scientist to determine original bacterial numbers during an experiment. Include any...

Extracted text: Using a diagram similar to those provided, describe how serial dilutions and the Standard Plate Count (SPC) allow a scientist to determine original bacterial numbers during an experiment. Include any calculations that would be relevant.


Extracted text: Volume of culture Start transferred for ** time serial dilution A B Volume of sterile 900 µL 900 µL 900 µL 900 µL water added to each tube prior to the transfer of bacteria Bacterial 9.9 mL culture broth at °C Volume of the diluted sample plated 20 µL 20 µL 20 µL 20 µL Plate A Plate B Plate C Plate D **Repeat this transfer at various time points into a new set of tubes