You are studying the uptake of L-leucine by epithelial cells of the mouse intestine. Measurements of the rates of uptake of L-leucine and severalof its analogs, with and without Na+ in the assay...


You are studying the uptake of L-leucine by epithelial cells of the mouse intestine. Measurements of the rates of uptake of L-leucine and several
of its analogs, with and without Na+
in the assay buffer, yield the results given in the table below. What can you conclude about the properties and mechanism of the leucine transporter? Would you expect L-leucine uptake to be inhibited by ouabain?


Uptake in presence of Na+ Uptake in absence of Na+<br>Substrate<br>Vmax<br>K+(mM)<br>Vmax<br>K†(mM)<br>L-Leucine<br>420<br>0.24<br>23<br>0.2<br>D-Leucine<br>310<br>4.7<br>5<br>4.7<br>L-Valine<br>225<br>0.31<br>19 0.31<br>

Extracted text: Uptake in presence of Na+ Uptake in absence of Na+ Substrate Vmax K+(mM) Vmax K†(mM) L-Leucine 420 0.24 23 0.2 D-Leucine 310 4.7 5 4.7 L-Valine 225 0.31 19 0.31

Jun 11, 2022
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