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1. Enzymes speed up chemical reaction in cells. As a result, energy usage is lowered such that this energy saved can be used for other chemical reactions in the cells.
2. Each enzyme can work optimally under a specific temperature and pH range. As temperature rises, reaction slows down , while ph reaches maximum midway. Lower than this or higher than this slows up reaction.
3. Main factors are i) Temperature, ii) pH and iii) concentration.
4. Most of the enzymes are proteins and hence their function is related to structure. They are a linear chain of amino acids, which lead to 3 dim structure. These regulate clotting of blood.
5. Amylaze catalysts the hydrolysis of starch into sugars. In mammals these start functioning in saliva itself to ease digestion.
6. WE used Maltose . Its pH is 6.5, temperature stability 0 to 50 degree celcius, isoelectric point is 5.2 and molecular weight 52000
7. DNS, known as Dinitrosalicylic acid reacts with reducing sugars and other sugar reducing molecules. As a result 3 amino 5 nydrosalicylic acid is formed. This acid can absorb light @540
nM
2. Experimental design:
a)
i) H0: There is linear association between temperature and absorbance at 540 nm
Ha: There is no...