The restriction enzymes AgeI and AvaI recognition sites are shown below. (NOTE, the arrows represent where the enzymes actually cut the DNA for each strand).
AgeI: 5’-ACCGGT-3’ AvaI: 5’-CCCGGG-3’
3’-TGGCCA-5’ 3’-GGGCCC-5’
Sequence 1: 5’-GGACCGGTCC-3’ Sequence 2: 5’-AACCCGGGTT-3’
3’-CCTGGCCAGG-3’ 3’-TTGGGCCCAA-5’
A. Write out the DNA fragments that are formed after cutting the above DNA sequences with:
Sequence I with AgeI :
Sequence 2 with AvaI:
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