The following data is from the analysis of a circular piece of DNA (plasmid). A. Use this data to generate a circular map of the plasmid. Pst I: 10 kb Bam HI: 10 kb Sal I: 4.5, 3, 2.5 Sal I + Pst I:...

The following data is from the analysis of a circular piece of DNA (plasmid).

A. Use this data to generate a circular map of the plasmid.

Pst I: 10 kb

Bam HI: 10 kb

Sal I: 4.5, 3, 2.5

Sal I + Pst I: 4.5, 3, 2, 0.5

Pst I + Bam HI: 7, 3

Sal I + Bam HI: 4.5, 2.5, 2, 1

B. You have purified the 3 kb Pst I to Bam HI fragment of DNA and have labelled the DNA for use in a Southern blot. If the double cuts are shown were run on a gel, transferred to nitrocellulose, and then analyzed by probe hybridization; which band or bands from each double-cut would be identified?