The effect of pH on the activity of an enzyme was examined. At its active site, the enzyme has an ionizable group that must be positively charged for substrate binding and catalysis to take place. The...

Extracted text: The effect of pH on the activity of an enzyme was examined. At its active site, the enzyme has an ionizable group that must be positively charged for substrate binding and catalysis to take place. The ionizable group has a pKa of 6.0. The substrate is negatively charged throughout the pH range of the experiment. At what pH = 9, the enzyme will be operating at answer is either Vmax, 1/2 Vmax or less than 1/2 Vmax (type in one of the 3 phrases as they are written here.) MacBook Air