Substrate Initial velocity Vo concentration [S] µM s-1 10 0.13 25 0.27 50 0.45 100 0.65 150 0.77 200 0.85 300 0.94 500 1.03 1. Calculate Vmax and Km??? The enzyme is dissolved at a concentration of 1...

Substrate<br>Initial velocity Vo<br>concentration [S] µM s-1<br>10<br>0.13<br>25<br>0.27<br>50<br>0.45<br>100<br>0.65<br>150<br>0.77<br>200<br>0.85<br>300<br>0.94<br>500<br>1.03<br>1. Calculate Vmax and Km???<br>The enzyme is dissolved at a concentration of 1<br>mg/ml and has a molecular weight of 50 kDa.<br>a) What is the turnover number of the enzyme?<br>b) What is the specificity constant of the<br>enzyme?<br>c) Is the enzyme operating near the maximum<br>rate possible, i.e. is the enzyme diffusion<br>controlled?<br>please show all working and formulas as I am<br>trying to learn. thank you<br>

Extracted text: Substrate Initial velocity Vo concentration [S] µM s-1 10 0.13 25 0.27 50 0.45 100 0.65 150 0.77 200 0.85 300 0.94 500 1.03 1. Calculate Vmax and Km??? The enzyme is dissolved at a concentration of 1 mg/ml and has a molecular weight of 50 kDa. a) What is the turnover number of the enzyme? b) What is the specificity constant of the enzyme? c) Is the enzyme operating near the maximum rate possible, i.e. is the enzyme diffusion controlled? please show all working and formulas as I am trying to learn. thank you

Jun 11, 2022

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Substrate Initial velocity Vo concentration [S] µM s-1 10 0.13 25 0.27 50 0.45 100 0.65 150 0.77 200 0.85 300 0.94 500 1.03 1. Calculate Vmax and Km??? The enzyme is dissolved at a concentration of 1...

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