Subject: Gene TechnologiesI need this gone by 06/05.Has to be more then 1000+
03/05/2022, 18:41 2022_Open-book assessment 1. https://rmit.instructure.com/courses/96347/assignments/663240 1/5 2022_Open-book assessment 1. Due Sunday by 23:59 Points 100 Submitting a file upload Attempts 0 Allowed attempts 2 Available 10 Apr at 9:00 - 15 May at 23:59 about 1 month Start Assignment BIOL2184 – Gene technologies Open-book assessment 1 (100 points, 30%) This assessment reflects your industry-related research Task: Genetic reprogramming of the valuable chemicals’ production in plants 2022_BIOL2184 Open-book assessment 1.pdf (h�ps://rmit.instructure.com/courses/96347/files/24171870/download?download_frd=1) Deadline: May 8th 23:59 10% penalty for each extra day Assessment brief, criteria, and rubric. This assignment outlines course learning outcomes (CLOs) requirements, which you will use during your future industry-related experience. On successful completion of this course, you should be able to: CLO 1. Understanding science: 1.1. You will demonstrate a coherent understanding of biological sciences by articulating the methods of science and explaining why current biological knowledge is both contestable and testable through further inquiry. 1.2. You will demonstrate a coherent understanding of biological sciences by explaining the role and relevance of biological sciences in society. CLO 2. Scientific knowledge: 2.1. You will exhibit depth and breadth of knowledge by demonstrating a well-developed understanding of biological sciences. https://rmit.instructure.com/courses/96347/files/24171870?wrap=1 https://rmit.instructure.com/courses/96347/files/24171870/download?download_frd=1 03/05/2022, 18:41 2022_Open-book assessment 1. https://rmit.instructure.com/courses/96347/assignments/663240 2/5 2.2. You will exhibit depth and breadth of knowledge by demonstrating that biotechnology has interdisciplinary connections with other sciences. CLO 3. Inquiry and problem-solving: 3.1. You will be able to critically analyse and solve problems in biotechnology by gathering, synthesizing and critically evaluating information from a range of sources. 3.2. You will be able to critically analyse and solve problems in biotechnology by designing and planning an investigation, including developing a testable hypothesis. 3.3. You will be able to critically analyse and solve problems in biotechnology by selecting and applying practical and/or theoretical techniques with technical competence in conducting field, laboratory-based, or virtual experiments. 3.4. You will be able to critically analyse and solve problems in biotechnology by collecting, accurately recording, interpreting, and drawing conclusions from scientific data. CLO 4. Communication: You will be an effective communicator of biological sciences by effectively communicating scientific results, information, or arguments using a range of modes (oral, written, visual) for a variety of purposes and audiences. CLO 5. Personal and professional responsibility: You will be accountable for individual learning and scientific work in biotechnology by being an independent and self-directed learner. Performance descriptors: For HD (80-100%) you need to achieve excellently all tasks following the instructions and guidelines listed above Criteria Ratings Pts Understanding of molecular cloning techniques and their applications 100.0 Pts 50.0 Pts 25.0 Pts 0.0 Pts 100 Good response Satisfactory response Unsatisfactory response No response provided Appropriate, relevant key points covered Understanding the task and the information but not all Not demonstrated an understanding of the topic No response 03/05/2022, 18:41 2022_Open-book assessment 1. https://rmit.instructure.com/courses/96347/assignments/663240 3/5 Open-book Assignment 1 key points are included information , often inaccurate or irrelevant 03/05/2022, 18:41 2022_Open-book assessment 1. https://rmit.instructure.com/courses/96347/assignments/663240 4/5 Criteria Ratings Pts 20 pts 20 pts 20 pts 20 pts Theoretical part Application of transgenic technologies in plant biotechnology and modern agriculture (appx 1-2 pages) 20 Pts Excellent The research topic has been clearly articulated and explained. The application of transgenic technologies in plant biotechnology and modern agriculture has been answered with related references 10 Pts Good Most but not all parts of the question have been answered sufficiently. The research topic has been articulated but may lack some clarity or detail. A good to a satisfactory number of references has been shown. 5 Pts Brief answer Most parts of the question have not been answered sufficiently. An explanation of the research topic is inadequate or has not been provided. References and examples concepts were shown, but some of them are irrelevant. 0 Pts Not satisfactory No attempt has been made to satisfactorily answer any parts of the question. Research part Design and describe a transgene for production of X in leaves (promoter, coding region, terminator). You can design more than one transgene 20 Pts Excellent All parts of the question have been clearly articulated and explained: Transgene and its key components for the production of X in leaves were clearly explained. The strategy is smart. The application of key transgene components was clearly explained. 10 Pts Good Understanding the task and the information but not all key points are included. One or two minor parts of the question are unanswered. The proposed strategy for transgene’s design is not the most efficient. The application of key transgene components was not clearly explained. 5 Pts Brief answer Several parts of the question are unanswered. An appropriate strategy has been proposed. Not demonstrated a good understanding of the topic. 0 Pts Not satisfactory No attempt has been made to satisfactorily answer any parts of the question. Research part Design and describe a vector that contains your transgene(s) 20 Pts Excellent The research topic has been clearly articulated and explained. All parts of the question have been answered: Vector and its key components for the production of X in leaves were clearly shown. The strategy is smart. The application of key transgene components was clearly explained. 10 Pts Good Understanding the task and the information but not all key points are included. One or two minor parts of the question are unanswered. The proposed strategy for transgene’s design is not the most efficient. The application of key transgene components was not clearly explained. 5 Pts Brief answer Several parts of the question are unanswered. An appropriate strategy has been proposed. Not demonstrated a good understanding of the topic. 0 Pts Not satisfactory No attempt has been made to satisfactorily answer any parts of the question. Research part Design a strategy for transformation, generation, and selection of the transgenic plants 20 Pts Excellent The research topic has been clearly articulated and explained. Design a strategy for transformation, generation, and selection of the transgenic plants were clearly explained. The strategy is smart. The application of key parts of the project was clearly explained. 10 Pts Good Understanding the task and the information but not all key points are included. One or two minor parts of the question are unanswered. The proposed strategy for transformation, generation and selection of the transgenic plants is not the most efficient and /or was not clearly explained. 5 Pts Brief answer Several parts of the question are unanswered. An appropriate strategy has been proposed. Not demonstrated a good understanding of the topic. 0 Pts Not satisfactory No attempt has been made to satisfactorily answer any parts of the question. 03/05/2022, 18:41 2022_Open-book assessment 1. https://rmit.instructure.com/courses/96347/assignments/663240 5/5 Total points: 100 Criteria Ratings Pts 20 pts Research part Design strategies to confirm your generated transgenic plant 20 Pts Excellent The research topic has been clearly articulated and explained. Design a strategy to confirm your generated transgenic plant was clearly explained. The strategy is smart. The application of key parts of the project was clearly explained. 10 Pts Good Understanding the task and the information but not all key points are included. One or two minor parts of the question are unanswered. The proposed strategy to confirm transgenic plants was not clearly explained. 5 Pts Brief answer Several parts of the question are unanswered. An appropriate strategy has been proposed. Not demonstrated a good understanding of the topic. 0 Pts Not satisfactory No attempt has been made to satisfactorily answer any parts of the question. RMIT Classification: Trusted BIOL2184 – Gene technologies Open-book assessment 1 (100 points, 30%) Open-book assessment 1 This assessment reflects your routine industry-related research: Task: Genetic reprogramming of the production of the valuable chemical in plants Project Your company grows plants that produce a valuable chemical X in floral petals (flowers). Genes responsible for the biosynthesis of this chemical are GeneXA, GeneXB, and GeneXC. The petal-specific transcription factor, TFX, regulates these genes. Problem: These plants cannot be commercially used for the large-scale production of X because: • they do not produce many flowers; • flowers are small and • the production is seasonal and short since these plants flower only in spring and flowering lasts just a few days. As a result, it is not economical to produce enough biomass of petals for the commercial production of chemical X. Your project To increase the production of the chemical X, your company decided to reprogram the expression of genes XA, XB and XC, re-directing the production of chemical X from flowers to leaves of the same plants. The rationales behind this decision are: • these plants have big leaves, • leaves’ growth is not seasonal and • leaves and flowers have the same genome, and all genes involved in X production exist in leaves but are not active. RMIT Classification: Trusted You are the lucky researcher who must run this project. You need to design a strategy to conduct this project. You need to: 1. Theoretical part: Describe an application of transgenic technologies in plant biotechnology and modern agriculture with examples (appx 1-2 pages): 20 marks Appx 3 examples. • Why are these papers interesting? • What has been achieved? • How?: briefly experimental part 2. Research part: • Design transgene(s) for the production of X chemical in leaves (e.g. promoter, coding region, terminator). Show a transgene as a map: 20 marks • Design and describe a vector that contains your transgenes. The vector should have a selectable marker for plants. Show vector as a map: 20 marks • Design a strategy for transformation, generation, and selection of the transgenic plants: 20 marks • Design strategies confirm your generated transgenic plant: expression of the transgene and the production of chemical X: 20 marks Your report: A 1000+ word review. You can add pictures and maps if required. Use paragraphs to separate different parts of the review. Show in bold critical components of the gene silencing mechanisms. You must clearly address a research topic ● The information presented must be accurate and logically organised ● You should demonstrate an innovative solution to an