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Skin Anatomy, Neurological Testing 1, and General Sensory Pathways (Lab Manual pg. 73) Lab Preparation · Review the anatomy of cutaneous tissue. · Review your lab atlas and the list of video links associated with this lab. · Follow your lab manual and complete the activities therein as it will guide your investigation. The lab manual is also the document that will inform the Lab Tests. Lab Objectives · Identify skin, hypodermis regions on models and slides. · State the common names and functions of cutaneous glands. · State the functions and locations of cutaneous sensory receptors. · Identify the cutaneous general sensory receptors. Having studied the CNS and the peripheral nerves directly associated with both the brain and spinal cord we now turn our attention to how the body senses stimulation. Sensory reception can be classified according to stimulus type, origin of stimulus, and receptor structure. This lab deals with general sensory structures and their reflex pathways while the following two lab activities focus on special sensory structures and their reflex pathways. In order to address any reflex pathway we must first address the nature of the stimulus that the body is responding to. Towards that end we revisit the Integumentary system, only this time we focus on specific mechanoreceptors, presence or absence of encapsulation, and location within the dermis. You will then explore the reflexive responses of four spinal reflex arcs. The last part of this lab addresses how the body is able to adapt to constant stimulus or resist adaptation dependent upon the nature of the stimulus. Review of the Integumentary System Video Link to The Integumentary System Video Link to The Science of Skin Cutaneous Accessory Structures Video Link to The Integumentary System Part II Video Link to Composite Skin Model No need to focus on this much detail regarding secretory products but this is a good description of the names and locations of the sebaceous and sudoriferous glands. Video Link to Meissner corpuscle, Pacinian corpuscle, Ruffini ending , Merkel disc This video discusses all the general sensory receptors you need to be familiar with. Focus on function and location. The details regarding adaptation and receptor field pertain to the two-point discrimination test and various tests regarding adaptation we will look at later in this activity. Ignore the discussion regarding A and B axons Video Link to Touch Receptors Blood Vessels of the Integumentary System Capillaries Arterioles & Venules Arteries & Veins Overview of Monosynaptic and Polysynaptic Reflex Pathways Video Link to Monosynaptic and Polysynaptic Reflexes NOTE: For the following reflexes focus on the stimulus and reflexive response. I will not ask about which specific nerve numbers are participating in the reflex arc. The most detail I would ask about is whether it is a spinal or cranial reflex. Once you go through these examples that distinction should be clear. Patellar (Knee-jerk) Reflex Video Link to Knee-jerk Reflex Video Link to Normal Patellar Reflex Exam Video Link to Patellar Reflex Test Achilles Tendon (Ankle-jerk) Reflex Video Link to Achilles Heel Tendon Reflex Test Withdrawal Flexor Reflex with Crossed-Extensor Component Video Link to Withdrawal/Flexor Reflex Video Link to Crossed-Extensor Description Plantar Flexion Reflex (Babinski sign) Video Link to The Plantar Flexion Reflex Cranial Nerve Reflexes The following video describes the procedures involved in assessing the 12 pairs of cranial nerves. Video Link to Cranial Nerve Examination NOTE: The cranial reflexes WILL NOT BE TESTED ON. I have included this video just for personal interest. Two-Point Discrimination Test (Can be tested at home) Video Link to Two-Point Discrimination Test Overview of Sensory Adaptation Video Link to Sensory Adaptation Video Link to Sensory Adaptation 2 Adaptation to Temperature Video Link to Fool Your Senses Radiating Pain Video Link to Ulnar Neuropathy at Elbow This video goes into much more detail than is required but the first half does a good job of describing the path of the ulnar nerve and the regions of the hand that it collects sensory information from. Video Link to Cubital Tunnel Surgery The first three minutes of this video offer additional explanation of the path of the ulnar nerve and the experience of tingling and numbness felt after striking your “funny bone”. There is no need to focus on the latter discussion regarding surgery techniques. Nerve Physiology Lab Activity (Complete this as you watch the Dissection Video) Part I Consider the normal behavioral responses of the frog as described in the chart below and seen in the video. Indicate in the chart below which surgical procedure you hypothesize will eliminate each reflexive behavior. Justify your hypothesis in the final column by identifying the CNS and/or PNS structure responsible for the normal behavior. Normal Frog Frog w/o cerebrum & diencephalon Frog w/o cerebrum, diencephalon, & brain stem Frog w/o cerebrum, diencephalon, brain stem, & spinal cord Identify the brain region, spinal cord, or cranial nerve that controls the normal behavior. Identify only ONE component unless otherwise instructed. Corneal reflex Eye blinks when cornea is touched Swimming Coordinated swimming movements using hind legs ? ? ? Hint: ID one brain division ? Posture Uses forelegs to maintain upright posture ? ? ? Hint: ID one brain division ? Breathing Normal bpm ? ? ? Hint: ID one brain division ? Tongue position Tongue lies flat on floor of mouth ? ? ? Hint: ID one cranial nerve ? Righting reflex Quickly rights itself when placed upside down ? ? ? Hint: ID one brain division & one cranial nerve ? Acid reflex Attempts to wipe acid away with leg swipe ? ? ? ? Withdrawal reflex Quickly flexes leg away from stimulus ? ? ? ? Pupillary Reflex Predictions How will the pupils react when exposed to acetylcholine? Type answer here. How will the pupils react when exposed to epinephrine? Type answer here. Part IIA While watching the video, mark the columns where the behavior was eliminated. After the video, fill out the additional columns indicating if this matched your hypothesis. Correct your explanation if necessary. Normal Frog Frog w/o cerebrum & diencephalon Frog w/o cerebrum, diencephalon, & brain stem Frog w/o cerebrum, diencephalon, brain stem, & spinal cord Normal Frog Write your explanation, modify if it was incorrect. Identify the brain region, spinal cord, or cranial nerve that controls the normal behavior. Swimming Coordinated swimming movements using hind legs ? ? ? ? Hint: ID one brain division ? Posture Uses forelegs to maintain upright posture ? ? ? ? Hint: ID one brain division ? Breathing Normal bpm ? ? ? ? Hint: ID one brain division ? Tongue position Tongue lies flat on floor of mouth ? ? ? ? Hint: ID one cranial nerve ? Righting reflex Quickly rights itself when placed upside down ? ? ? ? Hint: ID one brain division & one cranial nerve ? Acid reflex Attempts to wipe acid away with leg swipe ? ? ? ? ? Withdrawal reflex Quickly flexes leg away from stimulus ? ? ? ? ? Part IIB Organ and Neuromuscular Activity. Write in full sentences with correct grammar & spelling. Type answer here. What organ was removed from the frog? Type answer here. What was observed about this organ? Type answer here. Explain the reason behind this observation. Also, include the role of the CNS in an intact frog on this organ in your explanation. Type answer here. Sciatic Roots What happened when the sciatic roots were cut? Type answer here. Which of the three kinds of gated ion channels were stimulated by this? Type answer here. What happened when the sciatic roots were sprinkled with salt? Type answer here. Which of the three kinds of gated ion channels were stimulated when the sciatic roots were sprinkled with salt? Type answer here. Benzocaine Gel Which nerve was the focus of this experiment? Type answer here. What was observed upon electrical stimulation before the application of the gel? Type answer here. What was observed upon electrical stimulation after the application of the gel? Type answer here. Pupillary Reflex How did the pupils react when exposed to acetylcholine? Type answer here. How did the pupils react when exposed to epinephrine? Type answer here. 2