Sample Questions · Reference: Kilo (k)=1000 Centi (c)= 1/100 Milli (m) = 10-3 Micro (μ) = 10-6 Nano (n) = 10-9 pico (p) = 10-12 Molecular weight of double stranded DNA: 1 base pair = 660 g/mol Please...

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Sample Questions · Reference: Kilo (k)=1000 Centi (c)= 1/100 Milli (m) = 10-3 Micro (μ) = 10-6 Nano (n) = 10-9 pico (p) = 10-12 Molecular weight of double stranded DNA: 1 base pair = 660 g/mol Please write out answers to both sections, take a picture and upload. a. You want to ligate together a 1.2 kB DNA insert and a 7 kB DNA vector. You measure your vector concentration to be 18 ng/µL and your insert to be 11 ng/µL. Calculate what volume of vector you would use to get 0.1 µg of vector. b. You want to perform a 5:1 insert:vector molar ratio ligation. How many nanograms of insert would you add to your ligation? What volume of insert DNA would you add to your ligation? · For your DNA transformation experiment, you want to add 20 pg of your pUC19 miniprep DNA to the cells. The concentration of your pUC19 miniprep DNA is 442 µg/mL. Calculate what dilutions you would make and what volumes you would pipet to get 20 pg of your pUC19 miniprep DNA. Use serial dilutions instead of one large dilution. · You want to plate 100,000 live cells in one well of 6-well plate. You count cells using the TC20 counter. The result is: Total cells = 1.5 x 106 cells/ml Live cells = 9.7 x 105 cells/ml Calculate what volume of your trypsinized and resuspended cell sample you should add. Also calculate how much fresh media to add to the cells in the well of the 6-well plate (total working volume of 1 well= 3 ml). There will be 25 questions all will be related to CRISPR technique
Answered 2 days AfterMay 18, 2021

Answer To: Sample Questions · Reference: Kilo (k)=1000 Centi (c)= 1/100 Milli (m) = 10-3 Micro (μ) = 10-6 Nano...

Shweta answered on May 21 2021
153 Votes
Introduction:
We offer a list of good photo compounds and the oxidised forms in which simple neutron donation classes such as hydrogen or acetate groups on cyclopentadienyl-rings allow for fine tuning of Fe+III/Fe+II redox potentials from +0.403 V to 0.096 V versus a lithium met
al membrane. Time pairs of mannose and access to the quality compounds emulate human or monocyclic acyl Chuck schumer based binding affinities, resulting from either the evaporation of 2-aminobenzenthiol (L) with monoacetyl ferrocene in the molar ratio 1:1 or in the molar ratio 1:2 for formaldehyde ferocine have been prepared. HLa-Maf and H2Lb-Daf are the Schiff Base ligands that result from the polycondensation reaction. The sulphur in the thiol group, as well as the carbon atom in the imidazole ring of the ligands, chelate the binding sites to organic compounds. H2Lb-Daf is an often positively cargend schiff base binding site, whereas HLa-Maf is a benzoic sustainable economic growth or aliphatic enhance business performance binding sites. The connection of 1,1′-fc(SeLi)2, which is formed with 1,1′-fc(SeSiMe3)2 and n-BuLi, with authoritative of CpFe(CO)2I, yields new hetero compounds of the normal spinel 1,1′-fc[SeFeCp(CO)2]2 (fc = Fe(5-C5H4)2 (fc = Fe(5- At 50 °C and a 1:2 (metal/ligand) adsorbent dosage, photocatalytic Chairman processes of the organoiron diselenolate with bis-phosphines yielded 1,1′-fc(SeFeCp(PP)2. Chemical and physical and microscopy approaches were used to classify all of these groups. That addition of hydrogen and translate directly to the Cp-rings results in the classification and functional density theory (DFT) measurements of four distinct changed ferrocenes in the this article. His analysis offers an insight, however and in what degree the properties of ferrocene can be regulated or adjusted through the addition of a basic electrons donation device to the Cp-rings.
Lab Method:
In a 100 cm3 boiling tube 1.2 cm3 of 85 percent H3PO4 and 8 cm3 of acetic anhydride, add 0.2g of cyclohexanol. With a cling film plug in the flask's neck, shield the mix from humidity. Stir the solution for 3 minutes and 20 seconds in a steam bath. Drop the liquids from of the hot shower and dump them into a 250 cm3 flask partially of water. And use a capillary tube, stir the mixture the contents of the beaker. Continue adding NaHCO3 in small quantities, stirring constantly, until no CO2 is generated, i.e. no more "fizzing." (The presence of strong NaHCO3 on the side of the bottle is a sure sign that this level has also been achieved!). To catalyse the n - hexane from the water, chill the combination for 15 minutes in an ice bath. Vacuum clean the strong, then clean this with 50cm3 of water and let it dry for 15 minutes.
Infrared spectra of (a) pure ferrocene and (b) the substance...
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