An enzyme is discovered that catalyzes the chemical reaction A team of motivated researchers sets out to study the enzyme, which they callhappyase. They find that the kcat for happyase is 600 s−1 and...

An enzyme is discovered that catalyzes the chemical reaction A team of motivated researchers sets out to study the enzyme, which they call
happyase. They find that the kcat for happyase is 600 s⁻¹
and carry out several additional experiments.
When [Et] = 20 nM and [SAD] = 40 μM, the reaction velocity, V0, is 9.6μM s⁻¹. Calculate Km for the substrate SAD.