Although graphical methods are available for accurate determination of the Vmax and Km of an enzyme-catalyzed reaction, sometimes these quantities can be quickly estimated by inspecting values of V0...

Although graphical methods are available for accurate determination of the V_max
and K_m
of an enzyme-catalyzed reaction, sometimes these quantities can be quickly estimated by inspecting values of V₀
at increasing [S]. Estimate the V_max
and K_m
of the enzyme-catalyzed reaction for which the following data were obtained:

Extracted text: [S] (M) Vo (uM/min) 2.5 x 10-6 28 4.0 x 10–6 40 1 x 10-5 70 2 x 10-5 95 4 x 10-5 1 x 10-4 112 128 2 x 10-3 139 1 × 10-2 140