Regarding the production of monoclonal antibodies, it is CORRECT to state that: *A) Hybridomas are cells generated from the fusion of myeloma cells and cells from animals challenged with the antigen...

Regarding the production of monoclonal antibodies, it is CORRECT to state that: * A) Hybridomas are cells generated from the fusion of myeloma cells and cells from animals challenged with the antigen of interest that have the ability to produce antibodies and limited cell proliferation. B) All hybrid cells generated from the fusion of cells from the challenged animal and cells of the myeloma lineage will have the ability to produce antibodies reactive against the antigen of interest. C) The HAT selection medium contains aminopterin, which is an efficient inhibitor of the thymidine kinase metabolic pathway, thus eliminating myeloma cells that are not fused or fused with other cells of the same kind. D) The HAT selection medium contains hypoxanthine, which is an efficient inhibitor of the nucleotide synthesis salvage pathway, thus eliminating cells from the challenged animal that are not fused or fused with other identical cells. E) Hybridomas will produce antibodies with the characteristics of the animal challenged with the antigen of interest, being necessary to analyze each cell individually to select the best producing clones