Read the Spun Microhematocrit lab in your manual and watch the spun microhematocrit video. Using the measurements below complete the calculations for a normal male blood sample. We will use measurements from a millimeter ruler as opposed to using the chart seen in the video. Please follow the directions given in the lab and calculate the estimated percent hematocrit. There are more detailed instructions in your manual on page 32-34.
The hemoglobin can be determined by dividing the average hematocrit (Hct) by 3. When you calculate the hemoglobin look up the normal male hemoglobin numbers and decide if your results fall within those numbers. The results from the two samples are as follows:
Tube 1 Tube 2
(RBC) Length of Red Blood Cells 8mm. 24mm.
(TL)Total length of all 20mm. 40mm.
(Blood, buffy coat, plus plasma)
% Hematocrit(Hct)= RBC mm. / Total length mm. x100
Multiply above Hct x 100 % Hct 1 _____ % Hct2_____
Average Hct(%) = Hct1(%) +Hct2(%) divided by 2 % Hct 1 _____ + % Hct2____/2 =ave Hct _____
Estimated hemoglobin g/dL =Average Hct% divided by 3 Ave Hct% ______/3= __________
Does the estimated hemoglobin fall within the expected range of a normal adult male? (hint: hematocrit is not the same as hemoglobin)
yes / no (circle one)
*remember the hemoglobin is the average hematocrit divided by 3
*You may have to look up the normal ranges for a healthy male
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