Q1. a) Why do scientist use restriction enzymes to cut plasmids (commonly referred as vectors? b) Why it is important to know the location of restriction enzyme cut sites in these vectors (hint: it is...

Q1. a) Why do scientist use restriction enzymes to cut plasmids (commonly referred as vectors? b) Why it is important to know the location of restriction enzyme cut sites in these vectors (hint: it is important to know where it does not cut). c) Why will an enzyme that recognizes and cuts the sequence GATC cut your DNA more times than an enzyme that cuts at GTAGCAG sites? ( hint: this has everything to do with probability ) d) In your gel you ran both the uncut plasmid and the plasmid cut with one enzyme (in one place) . Why do these two lanes of your gel not look the same? e) What is the source of restriction enzymes? How do they function in nature? f) How can a mutation that alters a recognition site be detected by gel electrophoresis?