Primer 1 primes synthesis of the 5' to 3' strand (i.e. sense strand) of an oat cDNA/gene and encodes for first few amino acids appearing at the N-terminal end of the corresponding polypeptide. Primer...


Primer 1 primes synthesis of the 5' to 3' strand (i.e. sense strand) of an oat cDNA/gene and encodes for first few amino acids appearing at the N-terminal end of the corresponding polypeptide. Primer 156 primes synthesis of the 3' to 5' strand (i.e. antisense strand) of an oat cDNA/gene and encodes for the last few amino acids appearing at the C-terminal end of the polypeptide. The polypeptide encoded by this gene is 156 amino acids in length.




QUESTION:-Assume that a PCR using the combination of Primer 1 and Primer 156 plus oat genomic DNA resulted in the amplification of a DNA fragment of the 160bp larger than PCRs in (1) above. With respect to the structure to this relevant gene, what does this result indicate? Explain how you reached this conclusion



Jun 10, 2022
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