Practical report with results which i will provide
PowerPoint Presentation Guidelines on writing the mini project report Identification of an Unknown microorganism BIOL2416 & BIOL2417 Diagnostic Microbiology ❑ To understand the importance of identification of bacteria and its relevance to applied microbiology today. ❑ To be able to use the phenotypic tests learnt in previous practical sessions to identify bacterial isolates to species level using the RMIT identification tables. ❑ To understand the basis of the biochemical reactions tests and what they tell us about the metabolism of the investigated bacterium. Reading: ● Textbook: Microbiology with Diseases by Body System, 14th or 15th edition, Pearson. ● Any relevant peer-reviewed papers published in reputable journals. Learning Objectives FORMAT ➢Introduction and Aims ➢Materials and Methods ➢Table of Results (Deductive reasoning) ➢Discussion ➢References ➢Scanned results sheets and flowchart Word Limits BIOL2416 Undergraduates : 800 + 10% Excluding Tables, reference list, worksheet and flowchart. BIOL2417 Postgraduates: 1000 + 10% Excluding Tables, reference list, worksheet and flowchart. The Report Format In the introduction, provide the background information about this project including the following information: The importance and the benefits of identifying microorganisms. Highlight some rapid methods and automated equipment currently used in identifying unknown microbes. The aim of the project: • What did you intend to investigate in this study? • What did you expect to find out in this study? • What was the method(s) or approach(es) used in this study? Introduction What information is required in Materials and Methods? TABULATE the list of biochemical tests and media used in identifying your unknown. The table must have an appropriate title legend. Unknown Organism (code no.), growth medium, and growth conditions? Full name of the biochemical tests and key reagents used for each test with expected observations. (USE GERMM FOR THIS SECTION and include citation) Growth Media and incubation conditions – i.e. time, temperature and gaseous conditions (aerobic/ anaerobic). DO NOT include the following in Materials and Methods: Laboratory equipment like Bunsen burners, loops, swabs etc. The detailed method of each biochemical test. Just cite GERMM Any Results from your test Materials and Methods Materials and Methods- in text citation Citation: G.E.R.M.M and RMIT Practical Manual according to RMIT APA referencing. Course materials are not required in reference list. Please refer to the weblink for more information https://www.lib.rmit.edu.au/easy-cite/ • Diagnostic Microbiology Workbook’s author is RMIT Microbiology Teaching Team. • GERMM’s author is RMIT Microbiology Teaching Team https://www.lib.rmit.edu.au/easy-cite/ A short paragraph on the identity of your unknow organism. E.g. An unknown No “7” was identified as ”Staphylococcus aureus”. The unknown grew on HBA … and MAC… and colony morphology was.., catalase was .. Gram staining and microscopy techniques confirmed that this is a--------(Gram reaction, and bacterial cell shape). Furthermore, the identity of this unknown bacterium, as assessed by biochemical tests indicated in the table below (or Table no.) was confirmed to be --------(Genus- species). The RMIT ID Table number used for identifying your unknown must be included here. Deductive reasoning: Based on the flowchart, briefly explain how differential biochemical test results, and/or observation of the growth on a differential medium contributed to the final result to identify the unknow bacterium through genus to species levels, i.e. how did the test/growth results eliminate other genera and species, leading you to find the identity of your unknown bacterium. CITE “RMIT ID Tables for Bacteria” Explain any unexpected results obtained in identification of your unknown bacterium. E.g. if a lactose fermenter gave you a negative result for lactose fermentation. Why it happened? And suggest what should you do to verify it. Results Do not repeat your results here. In your discussion, select one clinical case from the literature where this bacterium was reported to be isolated. What was the clinical case? Name of disease and main symptoms. Use this clinical case as your starting point for discussion. Include the following information in the discussion: • What was the detection method used in the selected reference? • Compare the accuracy and sensitivity of your method to theirs. Furthermore: • BIOL2416 UG – You need to cite 2 additional resources (journal papers or authentic health authority cites) which reported clinical cases caused by this bacterium. Name the disease, and relevant body system/organ. • BIOL2417 PG– You need to cite 4 additional resources (journal papers or authentic health authority cites) which reported clinical cases caused by this bacterium. Name the disease, and relevant body system/organ. Discussion ❏ Make sure you include in-text citation correctly – refer to APA referencing guide. http://www1.rmit.edu.au/library/referencing-guides ❏ “No Numbering” listing in APA referencing list. ❏ Do not include any images that are subject to copyright. ❏ Plagiarism will be dealt with according to RMIT plagiarism policy and procedures. References http://www1.rmit.edu.au/library/referencing-guides Documents for submission: A single pdfs file which includes the following combined documents: 1. The full final written report + 2. A scanned copy of your signed results record + 3. A scanned copy of your final signed flowchart Due Date : BIOL2416: 23 Oct 2020, 23.59 pm BIOL2417: 1 Nov 2020, 23.59pm Scientific Report Submission: • This mini project grades include your hands on and bacterial identification techniques (RTC day 3-5), The written Scientific Report, the Recorded results sheets and flowchart • It is worth a total of 20% of the 50% marks allocated to the practical component hurdle of this course. The mini project assignment will be marked out of total 200 marks as explained below: 1. Your lab work (60 marks) & the 1st draft of your flow chart (40 marks). This grading will be completed by your demonstrator during the lab session =100 marks. 2. Final written report + final Flow chart = 100 marks Note: (Grading scheme detailed in the next two slides) Marking Scheme Marking Scheme: Lab work and 1st Flow Chart (100 marks) In class assessment by your demonstrator A. Lab work (60 marks) Criteria of assessment: Microbiology practical techniques (20 marks) Analytical thinking skill (20 marks) Organization of work (20 marks) B. Flow chart (40 marks) Criteria of assessment: Systematic, orderly flow of the identification steps (20 marks) Differential tests included in each step (10 marks) Expected genera/species are included in each step (10 marks) RMIT University© School/Department/Area 12 Marking Scheme: Final Written Report (100 marks) RMIT University© School/Department/Area 13 Scientific Report Sections Allocated Marks Introduction and Aim 15 Materials and Methods 15 Results 20 Final flow chart & working lab sheet 10 Discussion 20 References/In text citation (relevant references & correct format) 10 Grammar/Format 10 TOTAL 100 For inquiries on this assignment: ● Inquiries about the lab work and unknown identification during the lab session: Ask your demonstrator ● General inquiries in person after the lab session: Talk to Dr. Foong Yong (Stacey). ● Inquiries bout writing and submitting the report: Contact Dr. Istivan or Dr. Yong in person or by email. Guidelines on writing the mini project report Identification of an Unknown microorganism BIOL2416 & BIOL2417�Diagnostic Microbiology Learning Objectives The Report Format Introduction Materials and Methods Materials and Methods- in text citation Results Discussion References Scientific Report Submission:� Marking Scheme Marking Scheme: Lab work and 1st Flow Chart �(100 marks) Marking Scheme: Final Written Report (100 marks) For inquiries on this assignment: Table 1: Characteristics of Unknown TEST Results/Observation Gram stain GPR, Cockeye Growth of HBA 2-3 mm, grey, round, condensed, raised Growth of MAC 1-2 mm, light pink, small circles, condensed, convex Catalase Positive Oxidase NA Tube Coagulase Negative Slide Coagulase Negative Urease Positive DNAsc Negative R/S Novobiocin Resistant R/S Polymyxin B Susceptible