Pls help do this, would prefer anyone good at labs or Preeti. thank you PRAXX TA: Abstract: An abstract is a one-paragraph summary of your report. It should include (in this order) the background of...

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Answer To: Pls help do this, would prefer anyone good at labs or Preeti. thank you PRAXX TA: Abstract: An...

Preeti answered on Mar 16 2022
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UTSC Journal of Plant Stress
BIO A02 WINTER - 2022; 1(1): 1-6
Insert Principal Author’s Name/Student Number
                                    
Presence of Heavy metals in soil affecting the morphology o Arabidopsis thaliana
1Dept. of Biological Sciences, University of Toronto Scarborough, Toronto, Canada
UTSC BIOA02 Lab PRAXX
PRAXX TA:
Abstract: Presence of heavy metals in soil can occur natu
rally or artificially for example through human activities such as mining, etc. The natural presence of heavy metals in soil is generally considered safe because it is present in low amount. However, human activities result in higher amount of heavy metals in soil which can be toxic to plants and may harm the plans and decrease the agriculture production. To study this, we selected four strains of Arabidopsis thaliana because of its small life cycle Col-0, Lov-1, Ws-2 and Santa Clara. The effect of the heavy metal upon the morphologic characters such as height and weight of these mutant strains were studied. After 4 weeks, the treated group of plants were watered with 8 mM of copper sulfate solution instead of the tap water. It was seen that metal toxicity doesn’t affect Arabidopsis and hence, it is not the right model to study the metal stress of plants.
Keywords: Arabidopsis thaliana, Heavy metal, Copper sulfate, Mutants, Stress, Response
Introduction
The effect of any environmental stress such as presence of heavy metals, temperature, salt concentration, drought etc. can be seen on plant growth , morphology, etc. (Harb et al., 2010). Presence of heavy metals in soil can occur naturally for example, through weathering of soil parent material or artificially for example through human activities such as mining, petrochemical industries, fertilizers, improper waste material etc. The natural presence of heavy metals in soil is generally considered safe because it is present in low amount. However, human activities result in higher amount of heavy metals in soil which can be toxic to plants and may harm the plans and decrease the agriculture production. To study the effect of heavy metal on plants, we selected Arabidopsis thaliana as our model organism because of its small life cycle. We selected four mutant strains of A.thaliana; Col-0, Lov-1, Ws-2, and Santa Clara. Col-0 is a laboratory strain whose genome has been completely sequenced and it has relatively low levels of seed dormancy. Lovvik-1 or Lov-1 is natural accession of Arabidopsis, which was first reported in Lovvik, Sweden. Similarly, Wassilewskija (Ws-2) is a natural accession of Arabidopsis that was originally collected in Wassilewskija, Russia. Santa Clara is also a natural accession of Arabidopsis that was originally collected in Santa Clara, CA, USA. In this study, 27 samples for both control and plants grown in the presence of heavy metal were selected for each strain and the height and weight of the plants were measured after 7 weeks. We concluded that metal toxicity doesn’t affect the Ws-2 and Santa Clara and has only affected the Col-0 and Lov-1.
Materials and Methods
The TA collected the seeds from the source lab in two 1.5ml centrifuge tubes. One tube was taken for control plant groups (which will be grown normally) and another for experimental or treated plant groups, which were grown in presence of heavy metals. For sterilization, the seeds were washed twice with 0.5 ml of 30% bleach and incubated for 3 minutes. Then, the bleach was removed, and the seeds were washed with 200μl of distilled water twice. After sterilization, 100 μl of cooled 0.1% agarose was added to seeds and mixed gently. 1ml Pipette tips were used to dot the agarose-seed solution on MS agar plates. The plates were wrapped in aluminium foil and kept in dark at 4oC for 3 days. Then, the plates were transferred to growth...
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