Peruse the PowerPoint and video demonstration for the formal lab activity. Enzymes Lab (online version) - Formal Lab Experiment (BIO181).pptx download Guidance Videos: Demonstration -...

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Peruse the PowerPoint and video demonstration for the formal lab activity.



Enzymes Lab (online version) - Formal Lab Experiment (BIO181).pptx

download



Guidance Videos:



Demonstration -
https://www.loom.com/share/3ae1a673816b42c7a6f4811e09ac5bc5(Links to an external site.)



How to Create a Line Graph in Excel -
https://www.loom.com/share/c6e9aac7498a48c0afe6444a0836ec8c(Links to an external site.)


Papers must be typed and double-spaced (other papers will be returned to thestudent ungraded). You may want to consult with tutors at the language arts building for othersuggestions on style and have someone you know proof read it. Each student must writehis or her own report (see information about plagiarismin the syllabus and its online references).


Please see the grading rubric for more details -->Rubric for grading BIO 181 laboratory reports.pdf

download


More assistance with the lab report can be found here -->Formal Lab - formatting guidance (enzymes).docx

download


APA Style Guide -->APA 6th Edition Style Guide.docx

download
(Note: general format shown in guidance document above is also okay)




Your report should conform to the standard format of a scientific journal that includes:Abstract,Introduction,Methods,Results,Discussion,Acknowledgments, andLiterature Cited. Use these terms as headings at the start of each section; they provide anoutline for your paper. You may want to break up each section and use additional subheadings.Type your name and an informative Title at the top of the first page. Suggestedlength: 4-5 pages.





  • Abstract: The abstract summarizes the rest of the paper: hypothesis & key findings.Introduction: This section informs the reader why you made the study. What observation or question stimulated you to perform these experiments? What backgroundinformation relates to the question? Be sure to write a clear, explanatory hypothesis thatyou tested. Finally, provide clear predictions of your hypothesis that will be addressed by your study.


  • Introduction:Provides information about thebackgroundand brieflyexplains the scientific conceptsto be examined in the laboratory exercise. Clearly states theobjective or purposeof the laboratory exercise. Also,effectively presentshypothesisand discusses the reasoning behind it. It is a good idea to specifically label the hypothesis as a subsection of this section here by hitting 'return' and typing,Hypothesis- , before writing it to make it clear to the reader what you are specifying as your hypothesis.


  • Materials and Methods: What did you do? How did you test your hypothesis? What materials wereused, at what temperatures, and why? What were your control groups and controlledvariables? This section should provide enough information to allow the reader to replicateyour study. Write this section and the results in the past tense.


  • Results: What did you find (measure, count)? You need to present your data clearly; however, too much detail can swamp the reader. One solution is to summarize the data in your written section (e.g. mean, sample size, range) and present the entire data set in a table and figure (i.e., graph). Write this section in past tense. Limit this section to the facts; avoid interpretations.


  • Discussion: Restate your hypothesis and key results and tell us: Do your results supportor reject your original hypothesis? If not, why? Were your methods appropriate for thisstudy? Why or why not? (refer to your controls). How could you improve the study, orwhat is the next step? Finally, what can you conclude? (hypothesis well tested andsupported/rejected).


  • Acknowledgments(optional section): Thank your colleagues, family or friends who helped (in the lab,reading your manuscript, etc.) and funding (e.g. grants, scholarships, parents).


  • Literature Cited: Scientific papers cite background studies for comparison to otherorganisms/systems and methods.You will need to cite your textbook and the lab manual from which this was adapted(two citations, minimum, then) for the lab and details of enzyme activity.


Peruse the PowerPoint and video demonstration for the formal lab activity.



Enzymes Lab (online version) - Formal Lab Experiment (BIO181).pptx

download



Guidance Videos:



Demonstration -
https://www.loom.com/share/3ae1a673816b42c7a6f4811e09ac5bc5(Links to an external site.)



How to Create a Line Graph in Excel -
https://www.loom.com/share/c6e9aac7498a48c0afe6444a0836ec8c(Links to an external site.)


Papers must be typed and double-spaced (other papers will be returned to thestudent ungraded). You may want to consult with tutors at the language arts building for othersuggestions on style and have someone you know proof read it. Each student must writehis or her own report (see information about plagiarismin the syllabus and its online references).


Please see the grading rubric for more details -->Rubric for grading BIO 181 laboratory reports.pdf

download


More assistance with the lab report can be found here -->Formal Lab - formatting guidance (enzymes).docx

download


APA Style Guide -->APA 6th Edition Style Guide.docx

download
(Note: general format shown in guidance document above is also okay)




Your report should conform to the standard format of a scientific journal that includes:Abstract,Introduction,Methods,Results,Discussion,Acknowledgments, andLiterature Cited. Use these terms as headings at the start of each section; they provide anoutline for your paper. You may want to break up each section and use additional subheadings.Type your name and an informative Title at the top of the first page. Suggestedlength: 4-5 pages.





  • Abstract: The abstract summarizes the rest of the paper: hypothesis & key findings.Introduction: This section informs the reader why you made the study. What observation or question stimulated you to perform these experiments? What backgroundinformation relates to the question? Be sure to write a clear, explanatory hypothesis thatyou tested. Finally, provide clear predictions of your hypothesis that will be addressed by your study.


  • Introduction:Provides information about thebackgroundand brieflyexplains the scientific conceptsto be examined in the laboratory exercise. Clearly states theobjective or purposeof the laboratory exercise. Also,effectively presentshypothesisand discusses the reasoning behind it. It is a good idea to specifically label the hypothesis as a subsection of this section here by hitting 'return' and typing,Hypothesis- , before writing it to make it clear to the reader what you are specifying as your hypothesis.


  • Materials and Methods: What did you do? How did you test your hypothesis? What materials wereused, at what temperatures, and why? What were your control groups and controlledvariables? This section should provide enough information to allow the reader to replicateyour study. Write this section and the results in the past tense.


  • Results: What did you find (measure, count)? You need to present your data clearly; however, too much detail can swamp the reader. One solution is to summarize the data in your written section (e.g. mean, sample size, range) and present the entire data set in a table and figure (i.e., graph). Write this section in past tense. Limit this section to the facts; avoid interpretations.


  • Discussion: Restate your hypothesis and key results and tell us: Do your results supportor reject your original hypothesis? If not, why? Were your methods appropriate for thisstudy? Why or why not? (refer to your controls). How could you improve the study, orwhat is the next step? Finally, what can you conclude? (hypothesis well tested andsupported/rejected).


  • Acknowledgments(optional section): Thank your colleagues, family or friends who helped (in the lab,reading your manuscript, etc.) and funding (e.g. grants, scholarships, parents).


  • Literature Cited: Scientific papers cite background studies for comparison to otherorganisms/systems and methods.You will need to cite your textbook and the lab manual from which this was adapted(two citations, minimum, then) for the lab and details of enzyme activity.


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Answered 2 days AfterJul 18, 2021

Answer To: Peruse the PowerPoint and video demonstration for the formal lab activity. Enzymes Lab (online...

Varun answered on Jul 21 2021
149 Votes
3
Objective: - Determining the Effect of Temperature on Reaction Rate of β-Galactosidase
Abstract: The influence of temperature on the hydrolysis of ONPG by β-galactosidase was examined utilizing enzyme assay. Temperature influences the paces of enzymatic responses in two d
ifferent manners. Higher temperatures increase the pace of the hydrolysis response, yet additionally increasing the pace of thermal denaturation of the enzyme. The impact of temperature on enzyme kinetics was concentrated by performing ONPG hydrolysis tests at 0, 10, 22, 37, 50, and 60˚C. The enzyme kinetics was seen to follow Arrhenius-type temperature reliance. The UV-Vis absorbance of converted ONG was utilized to assess the enzymatic activity of β-galactosidase at various temperatures. The maximum catalytic rate was seen at 37oC; afterward, activity tends to diminish with increasing temperature because of enzyme denaturation.
Introduction: Like most catalytic reactions, the pace of an enzyme-catalyzed response enhances as the temperature is increased. However, at higher temperatures the rate diminishes again because the enzymes become denatured and can at this point don't function. On account of enzyme-mediated responses, the catalytic rate increases with temperature to a most extreme level, then, at that point unexpectedly decline with an additional temperature rise. Since most proteins quickly become denatured at temperatures above 40°C, most catalytic determinations are completed to some degree underneath that temperature. A maximum action of enzymes is attained at the optimum temperature (Daniel, Danson, Eisenthal, Lee, & Peterson, 2008).
Enzymes/Catalysts speed up a chemical/biological response without themselves going through any perpetual chemical changes. They are neither utilized in the chemical response nor do they emerged in the final product. β-galactosidase, additionally called lactase or β-lady, is a group of glycoside hydrolase that catalyzes the hydrolysis of β-galactosides into mono-saccharides by breaking the glycosidic bond. One such substrate is O-nitrophenol-b-D-galactoside (ONPG), which when hydrolyzed produces galactose and O-nitrophenol (ONP). The compound ONP assimilates light at 420nm through the substrate ONPG doesn't. In this manner, the increment in light absorbance at 420 nm can be utilized to screen the catalytic activity of β-galactosidase when ONPG is utilized as a substrate at that specific temperature. The absorbance estimated for ONP specifically temperature is directly related to catalytic rate which is additionally used to affirm the enzymatic action of β-galactosidase (Carrara & Rubiolo, 1994).
             O-nitrophenol-b-D-galactoside + H2O O-nitrophenol +...
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