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2 Student Name: _____________________ Student ID: _____________________ Date Written: _____________________ (mm/dd/yy) Instructor: _____________________ Biology 30 (SCN3230) Unit 3: Cell Division, Genetics, and Molecular Biology 2016 Bow Valley College No part of this material may be reproduced by any means, including photocopying, without permission from Bow Valley College. Directions: • Complete all practice questions in the textbook as directed by your instructor before attempting this assignment. • The purpose of this assignment is to give you feedback before you write the unit exam. Submit this assignment, review your graded copy, try available practice exams, and then write the unit exam. • Print the assignment and write your answers as neatly as possible below the question. Illegible writing cannot be marked. Please write darkly if you are scanning or faxing. • Show all calculations and reasoning in the space provided. Show all aspects of your solution to earn full marks. The more work you show, the more I can tell you what you are doing correctly or incorrectly. • Present your responses in a well-organized manner to earn full marks. Complete all practice questions in the textbook as directed by your instructor before attempting this assignment. Complete your assignment independently. Updated: July 19, 2016 Created: Unknown Author(s): Lee Murray Reviewer(s): Lindsay Bonenfant Security: Official Curriculum Version: 3 00 Unit 3 Assignment Page 1 of 2 For full marks, your responses must address all aspects of the question. Descriptions and/or explanations of concepts must include pertinent ideas, diagrams, and calculations, where required. 1. Draw a flow chart or diagram to illustrate the process of producing a cloned woolly mammoth from a mammoth tusk marrow cell, elephant egg cell, and a surrogate elephant mother. Label the flow chart or diagram and for each step include the ploidy (2n or n) of the individual cells and the cloned mammoth. (4 marks) a. Dr. Ian Wilmut, an embryologist at the Roslin Institute in Edinburgh, led a group of scientists who cloned an adult sheep. In the procedure, cells were taken from the mammary tissue of an adult ewe (a female sheep). Using an electric current, these cells were individually fused with enucleated oocytes (eggs with the nuclei removed) from donor ewes. Most of the resulting embryos were implanted into surrogate ewes and allowed to develop to term. In July 1996, a cloned sheep, subsequently named Dolly, was born as a result of this procedure. She was the first mammal to be cloned using nuclear transfer from an adult cell. Wilmut's successful experiment, coupled with genetic engineering techniques, will result in new research and technology, using his techniques. Dolly's birth stimulated extensive discussion and debate among both scientists and non-scientists. A partially intact woolly mammoth has been discovered frozen in ice in Siberia. Some scientists hope to clone the woolly mammoth using a cell from the frozen marrow of the woolly mammoth's tusks, a donor elephant egg cell, and a surrogate elephant mother. Scientists in Russia and North America are searching for potential habitats suitable for mammoths. Unit 3 Assignment Page 2 of 2 b. Explain why the phenotype of the cloned mammoth would be similar to the woolly mammoth and not to the elephant egg donor or surrogate elephant mother. Compare and contrast how genotype and phenotype are determined in normal sexual reproduction with how they are determined in this type of cloning. (3 marks) 2. a. Circle the mode of transmission for the disorder in the pedigree? (1 mark) a. autosomal dominant b. autosomal recessive c. sex linked dominant d. sex linked recessive b. Complete the pedigree chart with the correct known genotypes under the respective individuals. Be sure to leave unknown genotypes as blank or incomplete. (eg; R_ , _ _ ) (3 marks) c. What is the probability of individuals III3 and III4 having a child that is a boy and having the disorder? (1 mark) d. Draw a Punnett square for the cross between individuals II2 and II5. (2 marks)