Mark the alternative that best explains the strategies for using tails and/or fusion proteins associated with the protein of interest. * a) fusion proteins have different functions, including...

Mark the alternative that best explains the strategies for using tails and/or fusion proteins associated with the protein of interest. * a) fusion proteins have different functions, including increasing the molecular weight of the protein of interest and thereby facilitating the process of protein purification by affinity chromatography. b) Histidine, c-Myc and GST tails have a dual function, since they can act both in reducing the formation of inclusion bodies and in facilitating the solubility of the protein of interest. c) the introduction of fusion proteins, such as Maltose-binding protein (MBP), at the C-terminus of the protein of interest has as main objective to increase the expression yield as they facilitate the protein aggregation process. d) the introduction of tails, such as c-Myc, in the C-terminus aims to facilitate the protein purification process through the affinity chromatography methodology.

Jun 11, 2022
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