Lab Report using redacted paper (Redacted Paper #2)
Goal:
Learn to evaluate scientific results and communicate those results in written form. (Like a lab report, except with someone else’s data.
Due: Before lab on 11/5
Value: 20 Points
Please review the Redacted Paper 1 lab handout for some tips on science writing. Everything in that handout applies to this assignment. I’m just not going to repeat it.
Your assignment:
Write the Introduction and Results sections of this paper.
· You should split your work into 3 sections. Use subheadings for these sections.
o Introduction/Background
o Figure 1B
o Figure 1C
· For the introduction, you need to provide the reader the background required to understand this paper. This should cover:
o The translocon
o Quality control mechanisms to prevent blockage of the translocon
o ER-resident RING-type ubiquitin ligases (these are the E3’s we discussed in clsss).
o Hrd1p
o ER stress
o Snf1p
o The last paragraph of the intro should be the overall logic for attempting these experiments.
· In the introduction, you should use as many references as you need. They should ALL come from peer-reviewed sources.
· You should have at least 3 references that are not already used in the Broshar paper. (You may also use references that are cited by Broshar and Rubenstein (the authors of this paper).
· The Figure 1 section should be written like the we discussed for the first Redacted paper assignment.
o Be sure to introduce the question being asked, the approach taken to answer it, and then the results of that experiment.
o Include experimental details if necessary to understand the logic. (for instance, why are they using cycloheximide?)
o Include the relevant results, but not EVERY result. Focus on the important things.
· Figure 2 – Should look like figure 1. Get to the “why” and then the “how” then the results.
· Summary paragraph: Finish things off with a summary paragraph. Draw conclusions from the results presented here. Refer back to important pieces of data that allowed the conclusion(s).
More specifics because I’m sure some of you will ask me these questions
· This paper should be a maximum of four pages. No less than 10 point font. There is no minimum. Write about the results.
· Make the paper double spaced. It is easier to grade.
· You do need references this time, and a works cited section
· You will submit the paper via Turnitin.
Questions to help guide your discussion of these results*. I suggest you record the answers as you discuss the paper.
1. Introduction:
- What specific proteins do the authors mention in the introduction? What do they do? (when starting your discussion of the paper, I recommend googling each gene just so you know what is going on).
b. What question is the author asking with this work?
c. Why is that question important?
2. Figures 1a
a. What is the purpose of figure 1a?
3. Figure 1b.
- What science question are the authors asking with the data presented here?
- How do the authors obtain the data?
- What does cycloheximide do?
- What type of gel is this?
- How do the authors see the bands?
- What are the numbers below the gel?
- What is Deg1*-Sec62?
- What is DTT and why is it being used here? (Hint: it is a ____________ control)
- What is “Vec” and why is this sample included? (Hint: it is a __________ control)
- What is the key result in this figure?
- Which lanes of the gel do you compare to arrive at that key result?
4. Figure 1c
- What question are the authors asking with this figure?
- What approach do they take to answer that question?
- What are the * and # symbols in the data?
- What is the Y Axis?
- What important results are shown here?
5. What overall conclusions can be made after considering the results posted here.
*Note: I do not want you to answer all of these questions in your paper. But to write your paper well, you will probably need to know the answer to all of these questions. Your paper should focus on conveying the results, and highlighting the important information that provides those results.