===================================================================== Acq. Operator : SYSTEM XXXXXXXXXXSeq. Line : 4 Sample Operator : SYSTEM Acq. Instrument : HPLC01 XXXXXXXXXXLocation : P2-B-01...

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It is a practical about HPLC I attached the results


===================================================================== Acq. Operator : SYSTEM Seq. Line : 4 Sample Operator : SYSTEM Acq. Instrument : HPLC01 Location : P2-B-01 Injection Date : 26/02/2020 16:46:55 Inj : 3 Inj Volume : 20.000 µl Sequence File : C:\Users\Public\Documents\ChemStation\1\Data\APC- 2020-02-26 16-33-38\APC-. S Method : C:\Users\Public\Documents\ChemStation\1\Data\APC- 2020-02-26 16-33-38\APC.M (Sequence Method) Last changed : 12/02/2020 15:01:04 by SYSTEM min0.5 1 1.5 2 2.5 mAU 0 50 100 150 200 250 VWD1 A, Wavelength=254 nm (004-P2-B1-solution 4.D) 1. 08 8 1. 51 3 1. 76 3 ===================================================================== Area Percent Report ===================================================================== Sorted By : Signal Multiplier : 1.0000 Dilution : 1.0000 Use Multiplier & Dilution Factor with ISTDs Signal 1: VWD1 A, Wavelength=254 nm Peak RetTime Type Width Area Height Area # [min] [min] [mAU*s] [mAU] % ----|-------|----|-------|----------|----------|--------| 1 1.088 BB 0.0849 680.23877 128.26404 26.6504 2 1.513 BV R 0.1017 1700.93396 269.18112 66.6391 3 1.763 VB E 0.0972 171.28217 25.22155 6.7105 Totals : 2552.45490 422.66671 Data File C:\Users\P...\ChemStation\1\Data\APC- 2020-02-26 16-33-38\004-P2-B1-solution 4.D Sample Name: solution 4 HPLC01 26/02/2020 16:50:00 SYSTEM Page 1 of 2 ===================================================================== *** End of Report *** Data File C:\Users\P...\ChemStation\1\Data\APC- 2020-02-26 16-33-38\004-P2-B1-solution 4.D Sample Name: solution 4 HPLC01 26/02/2020 16:50:00 SYSTEM Page 2 of 2 ===================================================================== Acq. Operator : SYSTEM Seq. Line : 1 Sample Operator : SYSTEM Acq. Instrument : HPLC01 Location : P2-A-01 Injection Date : 26/02/2020 16:26:21 Inj : 1 Inj Volume : 20.000 µl Sequence File : C:\Users\Public\Documents\ChemStation\1\Data\APC- 2020-02-26 16-25-10\APC-. S Method : C:\Users\Public\Documents\ChemStation\1\Data\APC- 2020-02-26 16-25-10\APC.M (Sequence Method) Last changed : 12/02/2020 15:01:04 by SYSTEM min0.5 1 1.5 2 2.5 mAU 0 50 100 150 200 250 VWD1 A, Wavelength=254 nm (001-P2-A1-solution 3a.D) 1. 08 7 1. 52 0 1. 76 7 ===================================================================== Area Percent Report ===================================================================== Sorted By : Signal Multiplier : 1.0000 Dilution : 1.0000 Use Multiplier & Dilution Factor with ISTDs Signal 1: VWD1 A, Wavelength=254 nm Peak RetTime Type Width Area Height Area # [min] [min] [mAU*s] [mAU] % ----|-------|----|-------|----------|----------|--------| 1 1.087 BB 0.0769 247.88562 50.82626 12.8879 2 1.520 BV R 0.0844 1444.21350 249.90549 75.0865 3 1.767 VB E 0.0985 231.30087 33.96264 12.0256 Totals : 1923.39999 334.69438 Data File C:\Users\P...ChemStation\1\Data\APC- 2020-02-26 16-25-10\001-P2-A1-solution 3a.D Sample Name: solution 3a HPLC01 26/02/2020 16:29:26 SYSTEM Page 1 of 2 ===================================================================== *** End of Report *** Data File C:\Users\P...ChemStation\1\Data\APC- 2020-02-26 16-25-10\001-P2-A1-solution 3a.D Sample Name: solution 3a HPLC01 26/02/2020 16:29:26 SYSTEM Page 2 of 2 ===================================================================== Acq. Operator : SYSTEM Seq. Line : 1 Sample Operator : SYSTEM Acq. Instrument : HPLC01 Location : P2-A-01 Injection Date : 26/02/2020 16:34:48 Inj : 1 Inj Volume : 20.000 µl Sequence File : C:\Users\Public\Documents\ChemStation\1\Data\APC- 2020-02-26 16-33-38\APC-. S Method : C:\Users\Public\Documents\ChemStation\1\Data\APC- 2020-02-26 16-33-38\APC.M (Sequence Method) Last changed : 12/02/2020 15:01:04 by SYSTEM min0.5 1 1.5 2 2.5 mAU 0 50 100 150 200 250 VWD1 A, Wavelength=254 nm (001-P2-A1-solution 3c.D) 1. 09 6 1. 51 6 1. 76 2 ===================================================================== Area Percent Report ===================================================================== Sorted By : Signal Multiplier : 1.0000 Dilution : 1.0000 Use Multiplier & Dilution Factor with ISTDs Signal 1: VWD1 A, Wavelength=254 nm Peak RetTime Type Width Area Height Area # [min] [min] [mAU*s] [mAU] % ----|-------|----|-------|----------|----------|--------| 1 1.096 BV 0.1078 1340.66760 203.78035 34.2346 2 1.516 VV 0.0850 1492.02014 255.96870 38.0995 3 1.762 VB 0.0989 1083.43103 158.13739 27.6659 Totals : 3916.11877 617.88644 Data File C:\Users\P...ChemStation\1\Data\APC- 2020-02-26 16-33-38\001-P2-A1-solution 3c.D Sample Name: solution 3c HPLC01 26/02/2020 16:37:53 SYSTEM Page 1 of 2 ===================================================================== *** End of Report *** Data File C:\Users\P...ChemStation\1\Data\APC- 2020-02-26 16-33-38\001-P2-A1-solution 3c.D Sample Name: solution 3c HPLC01 26/02/2020 16:37:53 SYSTEM Page 2 of 2 ===================================================================== Acq. Operator : SYSTEM Seq. Line : 2 Sample Operator : SYSTEM Acq. Instrument : HPLC01 Location : P2-B-01 Injection Date : 26/02/2020 16:38:50 Inj : 1 Inj Volume : 20.000 µl Sequence File : C:\Users\Public\Documents\ChemStation\1\Data\APC- 2020-02-26 16-33-38\APC-. S Method : C:\Users\Public\Documents\ChemStation\1\Data\APC- 2020-02-26 16-33-38\APC.M
solution-4c-acy5twdv.pdf solution-3a-3-3axyqjnb.pdf solution-3c-zav4iilq.pdf solution-4-a-4mmuicqz.pdf solution-4-b-zq0zhrqo.pdf
Answered Same DayMar 05, 2021

Answer To: ===================================================================== Acq. Operator : SYSTEM...

Jayageetha answered on Mar 07 2021
155 Votes
Sheet1
    Quantitative Analysis by High Performance Liquid Chromatography (Level 7 version)
    Name            e-mail you would like marks sent to
                                                Your final mark        63        %
    KU ID
    number            Put your answers in the GREEN boxes
    Aspirin
    tR for aspirin =                1.52    min                        Correct
                                                N(aspirin)    1796.8509242829    Mark    10    10
    Wh/2 for aspirin =                0.0844    min                                    0
                                                            0
    Efficiency N (aspirin) =                1797    (no units)        (10 marks)
    Caffeine
    tR for caffeine =                1.767    min                        Correct
                                                N(caff)    1782.831926615    Mark    10    10
    Wh/2 for caffeine =                0.0985    min                                    0
                                                            0
    Efficiency N (caffeine) =                1783    (no units)        (10 marks)
    Column length, L                50    cm
                                                Correct
    Plate height, H (aspirin)                0.0278    cm        (10 marks)                H(aspirin)    0.0278241514    Mark    10    10
                                                            0
                                                Correct            0
    Plate height, H (caffeine)                0.028    cm        (10 marks)                H(caff)    0.0280426248    Mark    10    10
                                                            0
                                                            0
    t0 (tartrazine)                1.087    min
                                                Correct
    Capacity factor (k') aspirin =                0.285    (no units)        (10 marks)                k(aspirin)    0.3983440662    Mark    2    2
                                                            0
                                                Correct            0
    Capacity factor (k') caffeine =                0.625    (no units)        (10 marks)                k(caff)    0.625574977    Mark    10    10
                                                            0
                                                            0
    Resolution (R) for Aspirin and Paracetamol
    tR for aspirin =                1.516    min                        Correct
                                                R    2.7    Mark    10    10
    Width at base-aspirin (W1) =                0.085    min                                    0
                                                            0
    tR for paracetamol =                1.762    min
    Width at base-paracetamol (W2) =                0.0989    min
    R for Aspirin/Paracetamol =                2.675    (no units)        (10 marks)                        Mark    10    10
                                                Correct
    Is this resolution satisfactory?                YES        Use dropdown box                    Answer    YES
                                (10 marks)
    Resolution (R) for Paracetamol and Caffeine
    tR for paracetamol =                1.513    min                        Correct
                                                R    2.5    Mark    10    10
    Width at base-paracetamol (W1) =                0.1019    min                                    0
                                                            0
    tR for Caffeine =                1.764    min
    Width at base-caffeine (W2) =                0.0985    min
    R for Paracetamol/Caffeine =                2.504    (no units)        (10 marks)                        Mark    10    10
                                                Correct
    Is this resolution satisfactory?                YES        Use dropdown box                    Answer    YES
                                (10 marks)
                                                Correct
    Aspirin weighed out                0.7    g        (10 marks)                Mass    0.7    Mark    10    10
                                                            0
                                                Correct            0
    Aspirin conc (soln 1)                16.12    mg per ml        (10 marks)                Conc(1)    14    Mark    5    5
                                                            0
                                                Correct            0
    Paracetamol weighed out                0.045    g        (10 marks)                Mass    0.045    Mark    10    10
                                                            0
                                                Correct            0
    Paracetamol conc (soln 2)                4.48    mg per ml        (10 marks)                Conc(1)    0.9    Mark    0    0
                                                            0
                                                Correct            0
    Caffeine weighed out                0.1    g        (10 marks)                Mass    0.1    Mark    10    10
                                                            0
                                                Correct            0
    Caffeine conc (soln 1)                2.3    mg per ml        (10 marks)                Conc(1)    2    Mark    5    5
                                                            0
                                                            0
                                                Correct            0
    Aspirin conc (soln 3A)                9.672    mg per ml        (10 marks)                Conc(3A)    0.06448    Mark    0    0
                                                            0
                                                Correct            0
    Aspirin conc (soln 3B)                12.9    mg per ml        (10 marks)                Conc(3B)    0.1612    Mark    0    0
                                                            0
                                                Correct            0
    Aspirin conc (soln 3C)                16.12    mg per ml        (10 marks)                Conc(3C)    0.3224    Mark    0    0
                                                            0
                                                Correct            0
    Paracetamol conc (soln 3A,B,C)                2.24    mg per ml        (10 marks)                Para Conc    0.0896    Mark    0    0
                                                            0
                                                Conc            0
                                                Ratio (3A)    4.3178571429    Mark    10    10
                                                            0
                                                Conc            0
                                                Ratio (3B)    5.7589285714    Mark    10    10
                                                            0
                                                Conc            0
    Sol'n    Aspirin conc    Para conc    Conc ratio    Aspirin peak area    Para peak area    Ratio peak area                    Ratio (3C)    7.1964285714    Mark    10    10
    3A    9.672    2.24    4.3    1492    1083    1.4    (70 marks for table)                            0
    3B    12.9    2.24    5.75    1444.2    231.3    6.2                    Peak area            0
    3C    16.12    2.24    7.2    1706.4696    172.45189    9.9                    Ratio (3A)    1.377654663    Mark    10    10
    4                1706.8    168.4    10.13                                0
                                                Peak area            0
                                                Ratio (3B)    6.2438391699    Mark    10    10
                                                            0
                                                Peak area            0
                                                Ratio (3A)    9.8953371865    Mark    10    10
                                                            0
                                                Peak area            0
                                                Ratio...
SOLUTION.PDF
SOLUTION.PDF

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