In this problem, just put the order of the intermediates on the pathway, starting with P and ending with Z and explain your reasoning. (So to get you started, notice the class 1 mutants—and there is only mutant in this class, which is mutant #5—won’t grow on minimal medium, but will grow if you give it substance Z. However, giving it substances, p, w, x, or y won’t help. This means that the gene that is mutated lies on the pathway in a place that the Z substances is to its right and all the other substances are to its left. The easiest next one to look at is the line with 2 pluses, and then the line with 3 pluses, and then the line with 4 pluses).
Extracted text: CHALLENGE PROBLEM 2 You are carrying out Beadle Tatum type experiments to analyze a metabolic pathway in Neurospora. You know that the precursor in the pathway is a molecule symbolized as P and that the product is a vitamin symbolized as Z. You are sure that the pathway from P to Z is linear and that the molecules W, X, and Y are intermediates. However, there may be other intermediates not yet identi- fied. You obtain 10 independent mutations that cannot grow on minimal medium supplemented with P, but can grow on minimal medium supplemented with Z. The 10 mutants fall into four classes that can grow (+) or cannot grow (-) on minimal medium supplemented with the nutrients W, X, or Y. The data are shown in the accompanying table. P W X Y Z Class I (mutant 5) Class II (mutants 1, 3, 4. 6, 7) Class III (mutant 2) Class IV (mutants 8, 9, 10) Draw a linear metabolic pathway with P on the left and Z on the right, in which each of the intermediates W, X, and Y is shown in the order in which it occurs in the metabolic pathway in the synthesis of Z from P. + +