If there was India Ink added to a culture of Paramecium caudatum, it would increase the average number of food vacuoles because India ink contains no sugar or nutrients and the formation of food...

If there was India Ink added to a culture ofParamecium caudatum,it would increase the average number of food vacuoles because India ink contains no sugar or nutrients and the formation of food vacuoles would be the first stage in the metabolic process when digesting nutrient-rich particles.

What would be the observed effects on the number of food vacuoles  of different concentrations such as 1% (v/v), 10% (v/v), and 25% (v/v) of India ink? Will it change over a duration of 30 minutes?