(Given a 30L of (liquid) bioreactor installed with 3 six-blade turbine (flat-blade) impeller (W/D=1/5), the diameter of impeller is 10 cm, diameter of tank DT = 50 cm , impeller rotation speed N = 150...

(Given a 30L of (liquid) bioreactor installed with 3 six-blade turbine (flat-blade)

impeller (W/D=1/5), the diameter of impeller is 10 cm, diameter of tank DT = 50 cm ,

impeller rotation speed N = 150 rpm, air aeration rate Fa = 30 L
min
, the density of the

culture media (ρL) is 1g cm3 ⁄ , dynamic viscosity of the culture media μL = 10−2
g
cm ∙ sec
.
E. coli with a qO2
of 10 mmol O2⁄(g cells ∙ h) is to be cultured; oxygen solubility in the

liquid CL = 2 mg O2/L. Saturated dissolved oxygen concentration (C*) in the liquid at 1atm

and 25°C is 7 mg/L.

a) What maximum concentration of E. coli can be sustained in this bioreactor under

aerobic conditions?

b) What concentration of cells could be maintained if pure oxygen was used to spare the

reactor?