Geneticists isolated six trains of E. coli (strains 1-6) whose lac operons had one of the following mutations (a-f): a. deletion of lacY b. oº mutation c. missense mutation in lacz d. inversion of the...


Geneticists isolated six trains of E. coli (strains 1-6) whose lac operons had one of the<br>following mutations (a-f):<br>a. deletion of lacY<br>b. oº mutation<br>c. missense mutation in lacz<br>d. inversion of the lac operon (which does not include the lacl gene)<br>e. superrepressor mutation<br>f. inversion of lacZ, Y, and Z, but not lacl, P, o<br>Focusing on the mutation in B:<br>a. what is the state of the lac operon (on, off, inducible, or constitutive)?<br>b. Provide an explanation using repressor, RNA pol, transcription, and the appropriate<br>regulatory region of the operon.<br>c. If a pDNA of another lac operon is inserted into the bacteria with a wild type/normal<br>copy of the DNA, how would this impact the transcription of the original mutant in B?<br>

Extracted text: Geneticists isolated six trains of E. coli (strains 1-6) whose lac operons had one of the following mutations (a-f): a. deletion of lacY b. oº mutation c. missense mutation in lacz d. inversion of the lac operon (which does not include the lacl gene) e. superrepressor mutation f. inversion of lacZ, Y, and Z, but not lacl, P, o Focusing on the mutation in B: a. what is the state of the lac operon (on, off, inducible, or constitutive)? b. Provide an explanation using repressor, RNA pol, transcription, and the appropriate regulatory region of the operon. c. If a pDNA of another lac operon is inserted into the bacteria with a wild type/normal copy of the DNA, how would this impact the transcription of the original mutant in B?

Jun 11, 2022
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