From the following information determine the amino acid sequence of a peptide.
N-terminal Edman gives PTH-Alanine
C terminal carboxypeptidase treatment, no observable reaction
Trypsin cleavage gives three products
1. Arg
2. Peptide containing Ala, Lys
3. Peptide containing Asp, Met, Phe, Pro
Mild Chymotrypsin cleavage gives 2 peptides
1. Peptide containing Asp, Pro
2. Peptide containing Ala, Arg, Lys, Met, Phe
CNBr cleavage gives 2 peptides
1. Peptide containing Ala, Arg, Lys and homoserine
2. Peptide containing Asp, Phe, Pro
You must supply the answer as the 3-letter amino acid sequence from N-terminus to C-terminus in the form (you must use dashes, not spaces between the amino acids) for example;
Met-Thr-Glu-Trp