From the following information determine the amino acid sequence of a peptide. N-terminal Edman gives PTH-Alanine C terminal carboxypeptidase treatment, no observable reaction Trypsin cleavage gives...

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From the following information determine the amino acid sequence of a peptide.

N-terminal Edman gives PTH-Alanine


C terminal carboxypeptidase treatment, no observable reaction


Trypsin cleavage gives three products


1. Arg


2. Peptide containing Ala, Lys


3. Peptide containing Asp, Met, Phe, Pro


Mild Chymotrypsin cleavage gives 2 peptides


1. Peptide containing Asp, Pro


2. Peptide containing Ala, Arg, Lys, Met, Phe


CNBr cleavage gives 2 peptides


1. Peptide containing Ala, Arg, Lys and homoserine


2. Peptide containing Asp, Phe, Pro


You must supply the answer as the 3-letter amino acid sequence from N-terminus to C-terminus in the form (you must use dashes, not spaces between the amino acids) for example;


Met-Thr-Glu-Trp



Answered Same DayDec 22, 2021

Answer To: From the following information determine the amino acid sequence of a peptide. N-terminal Edman...

David answered on Dec 22 2021
127 Votes
N-terminal degradation of the amino acid sequence of the peptide gives PTH-Alanine. Thus in
there
is Alanine in the N-terminal end of the peptide.
There is no observable reaction with C terminal carboxypeptidase.C terminal carboxypeptidase
A cleaves amino side of c-terminal amino acid except arginine, lysine, methionine and proline.
As there is not any reaction with carboxypeptidase , then there must be any one of the mentioned
amino acid in the c-terminal end.
Trypsin cleavage gives three...
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