automated sequencing is used are given a printout of the sense strand of your DNA. The printout is linked. The first thing you need to do is use the correct reading frame. Having done this, the next thing to do is to write out the mRNA sequence using this sense strand reading frame. The last thing to do is to translate the sequence
Extracted text: File: gvt11b11.y1 Machine: Cochise-1414-017 Lane: 93 Primer: DT3730POP7{BDV3).mob Comment: 1778D3 Spacing: 15.06 Signal: C: 1728 A: 3254 G: 2538 T: 2619 Bases: 903 Gel name: Gel start date: 3 Jan 2004 Gel start time: 4:123 Matrix 10 20 30 40 50 60 70 80 NNACTCA TCTGGTGGA T TC C TA TC CTG AC A AG TG ATGTG CAAA C TG G TA ACTC TG AG GCAG A TAAC CA G GG CA AA AAGGTG TATAAG 100 120 90 TAAA ACA AAGCCTAAC T TG GAAGA AAGAGAGAAÄAAGT TCAGTT TTACACA TCTTTA TA TGAAGCAGA AG TC CGGA AA A TCA TT TA AAGAT 110 130 140 150 160 170 180 190 200 210 220 230 240 250 260 TATTCTAT TA ATGTCCTGT TA TA TT TG T CATATTCA TA CAGT TG TCACAGTA TA T T TCAAAC CA AC TG TTTAAA A ACAA AC TG AAA TA AA 270 AAATTTAAA TACCCT TA TG TA AA ATAG GCT TC CCTGGTG GCTCAG GG GTAAA AA ACTCGCCCG C CA ACGCAGGAGATGTAGAT TTGATC CC T 280 290 300 310 320 330 340 350 380 360 GG GT TAG G A AG ATCCC CTGGAGA AG GAAATGAA AAAC CACTCTAG TAT TCT TGC CTG GG A A AT C CCA TG GACAG AG GAGCGTGGAGG GC 370 390 400 410 420 430 440 TACAGTCCATGGGAGTCGCAAA AGAGT TG GACATG ACTAAACAACAACATATAAAATAACCT TACTC CATA ATGTCAAACT TATGTCACAC 450 460 470 480 490 500 510 520 530 550 580 590 600 610 620 540 AAA ATGCAA AGT TCT TACATCTAT TA ACTTTTATG GT TA AATATAACCTAATGCACTGTTT TATACAGCA ACAACTA CTTTTT TATTT TAAA 560 570 630 710 720 680 TG GTGAAAAAT TAGTGAACAGAGG GTCTCAGAGATGCTAAACT TATGACT TATAAT TAACAAGATGT TÄTAGTTCTAGACAT TTTTGATAT 640 650 660 670 690 700
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