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57470
Worksheet 2
1) The strH structural gene is 1157 base pairs in size. As mentioned in the worksheet, the gene that encodes for strH is from 283980 to 285137 base pairs. Structural genes are the genes that code for functional proteins. Thus, the structural gene of strH is 1.157 kb.
2) Each amino acid is made up of a combination of three (out of total 4) base pairs like ATG codes for Methionine which is a start codon. Thus, a processed strH protein would have about 383 amino acids. The start and stop codon would not be coding for an amino acid in the processed protein.
3) The single letter amino acid sequence for the below encoding nucleotides is as follows:
ATG CAG ACC AGC GCG CAT CCC GCT
Mf Q T S A H P A
4) Translation of sequence after error
Sequence W: ATG CAG ACT AGC GCG CAT CCC GCT
Mf Q T S A H P A
Sequence X: ATG CAG ACC AGC GCG CAA TCC CGC T
Mf Q T S A Q S R
Sequence Y: ATA CAG ACC AGC GCG CAT CCC GCT
There will be no translation as the start codon is not present.
Sequence Z: ATG CAG ACC AGC GCG GAT CCC GCT
Mf Q T S A D P A
5) Impact of replication error on protein structure and function of strH –
Sequence W: this sequence is same as the original sequence as ACC was replaced by ACT which code for the same amino acid.
Sequence X: the last three amino acid of the sequence H, P & A have been replaced by the Q, S & R amino acid. H is positively charged while P and A...