Estimation of parameters in the Monod model. From measurements of the residual glucose concentration in a steady-state chemostat at various dilutionrates, you can find the following results: Calculate...

Estimation of parameters in the Monod model. From measurements of the residual glucose concentration in a steady-state chemostat at various dilutionrates, you can find the following results:

Calculate by linear regression the parameters in the Monod model. Are any of the data points suspect? If you want to check the value of m max determined above, increase the dilution rate in the chemo    stat to D = 1.1 h1. This results in a rapid increase of the glucose concentration in the medium. After a while, s >> Ks. The result of the change in  6Benzoic acid as well as citric acid and lactic acid are used to preserve food, exactly because of their inhibition of microbial growth. In acidified food (it could be a soft drink or a fruit juice, or the pH may start to decrease through incipient growth of spoilage bacteria) much of the benzoic acid is on the un dissociated form (pKa = 4.1), and the un dissociated acid freely diffuses into the cell. Inanaerobic yeast cultivation, growth of the yeast almost stops when pH reaches 5, but the ethanol yield increases in a frantic effort of the yeast cell to acquire enough ATP to expulse the protons after the acid has dissociated at the intracellular pH of close to 7. Lactic acid (pKa = 3.88) has the same effect.

dilution rate is a decrease in the biomass concentration, and during the wash-out you measure the biomass concentration as a function of time, and obtain the following results:

Determinem_max
from this experiment. Discuss the applied method [see also Eseneret al.(1981c)].