Consider the emission light path of a fluorescence microscope. Assume 500 photons fall in a wavelength band for which the dichroic beam splitter has a transmission of 90%, the emission filter has a...


Consider the emission light path of a fluorescence microscope. Assume 500 photons fall in a wavelength band for which the dichroic beam splitter has a transmission of 90%, the emission filter has a transmission of 95%, and the camera has a quantum efficiency of 60%. How many photons will be detected in the camera?



May 03, 2022
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