Consider an infinity-corrected microscopy system consisting of an objective and a tube lens with an image focal length of 200 mm. Assuming that the medium associated with the image side of the tube lens is air and the magnification of the microscopy system is 100×, determine how the distance so to an in-focus object changes if the immersion medium of the objective is changed from air, to water, to oil. How will these distances change if the magnification of the microscopy system is changed to 40×?
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