Consider a negatively charged spherical particle of radius a bearing a charge, Qs, suspended in a pure dielectric fluid (containing no ions). When subject to a uniform electric field, E~N, the...



Consider a negatively charged spherical particle of


radius a bearing a charge, Qs, suspended in a pure dielectric


fluid (containing no ions). When subject to a uniform


electric field, E~N, the particle will translate under the influence of the electric force acting on it. The induced particle


motion refers to electrophoresis, which has been widely used


to characterize and purify molecules and colloidal particles.


The net electrical force on the charged particle will simply be


F


~


E 5 QSE~N. As soon as the particle starts to move under the


influence of this electric force, it encounters an oppositely


directed fluid drag force.


(a) Under the Stokes flow regime and neglecting the gravitational force and the buoyancy force acting on


the microparticle, derive an expression to calculate the


particle’s steady-state translational velocity.


(b) Based on the above results, explain why electrophoresis


can be used to separate biological samples.


(c) Calculate the translational velocities of two particles


of radius a 5 1 μm and 10 μm using Qs 5 210212 C,


EN 5 1000 V/m, and μ 5 1023 Pa s.



May 26, 2022
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