Conditional mutations are useful for genetic analysis of essential processes. For example, temperaturesensitive cell-cycle mutations in yeast do not divide at 37C (nonpermissive temperature) but will...


Conditional mutations are useful for genetic analysis of essential processes. For example, temperaturesensitive cell-cycle mutations in yeast do not divide at 37°C (nonpermissive temperature) but will divide at 30°C. An alternative type of conditional mutation is a cold-sensitive mutation in which the nonpermissive temperature is low (23°C). List the steps you would go through to isolate cold-sensitive cell-cycle mutants of yeast.

May 08, 2022
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