Conditional mutations are useful for genetic analysis of essential processes. For example, temperaturesensitive cell-cycle mutations in yeast do not divide at 37°C (nonpermissive temperature) but will...

Conditional mutations are useful for genetic analysis of essential processes. For example, temperaturesensitive cell-cycle mutations in yeast do not divide at 37°C (nonpermissive temperature) but will divide at 30°C. An alternative type of conditional mutation is a cold-sensitive mutation in which the nonpermissive temperature is low (23°C). List the steps you would go through to isolate cold-sensitive cell-cycle mutants of yeast.