Complete this Master Mix table for 8 DNA samples, a positive control, negative control, and an extra reaction for pipetting error. Show your work. Master Mix Conc. Of Total μL µL/Rxn (total per tube)...


Complete this Master Mix table for 8 DNA samples, a positive control, negative control, and<br>an extra reaction for pipetting error. Show your work.<br>Master Mix Conc. Of<br>Total μL<br>µL/Rxn<br>(total per<br>tube)<br>Final<br>Number of<br>Stock<br>Conc.<br>Reactions<br>needed for<br>solution<br>master mix<br>PCR buffer<br>50X<br>1X<br>Water<br>DNTP mix<br>100 mM<br>200 µM<br>MgCl2<br>Forward<br>24 mM<br>2.5 mM<br>2 μΜ<br>0.1 μΜ<br>primer<br>Reverse<br>2 μΜ<br>0.1 μΜ<br>primer<br>Taq<br>polymerase<br>DNA<br>5 U/uL<br>0.03 U/ µL<br>1 μL<br>60 μL<br>Total<br>volume of<br>the entire<br>reaction<br>(µL)<br>

Extracted text: Complete this Master Mix table for 8 DNA samples, a positive control, negative control, and an extra reaction for pipetting error. Show your work. Master Mix Conc. Of Total μL µL/Rxn (total per tube) Final Number of Stock Conc. Reactions needed for solution master mix PCR buffer 50X 1X Water DNTP mix 100 mM 200 µM MgCl2 Forward 24 mM 2.5 mM 2 μΜ 0.1 μΜ primer Reverse 2 μΜ 0.1 μΜ primer Taq polymerase DNA 5 U/uL 0.03 U/ µL 1 μL 60 μL Total volume of the entire reaction (µL)

Jun 10, 2022
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