Comparison of the genomes of a wild-type bovinetuberculosis strain of Mycobacterium bovis and contemporary M. bovis-derived bacillus Calmette-Gue´rin (BCG) vaccine strains with that of a virulent...

Comparison of the genomes of a wild-type bovinetuberculosis strain of Mycobacterium bovis and contemporary M. bovis-derived bacillus Calmette-Gue´rin (BCG) vaccine strains with that of a virulent reference strain of M. tuberculosis indicated that almost 100 M. tuberculosis genes were found to be ‘‘missing’’ from all of the M. bovis and BCG isolates that were used for making the vaccines. Describe how you might isolate and identify those genes that are unique to M. tuberculosis.