Cell Circularity Data. In the lab of Dr. Todd McDevitt at Georgia Tech, researchers wanted to elucidate differences between the “static” and “rotary” culture of embrionic bodies (EBs) that were formed...

Cell Circularity Data. In the lab of Dr. Todd McDevitt at Georgia Tech, researchers wanted to elucidate differences between the “static” and “rotary” culture of embrionic bodies (EBs) that were formed under both conditions with equal starting cell densities. After 2, 4, and 7 days of culture, images of EBs were acquired using phase-contrast microscopy. Image analysis software was used to determine the circularity (defined as 4π(Area/Perimeter2)) of each EB imaged. A total of n = 325 EBs were analyzed from three separate plates for both static and rotary cultures at the three time points studied. The circularity measures were used to examine differences in the shape of EBs formed under the two conditions as well as differences in their variability. The data set circ.dat|mat consists of six columns corresponding to six treatments (2d, rotary), (4d, rotary), (7d, rotary), (2d, static), (4d, static), and (7d, static). Note that this is not an example of multivariate data since the columns are freely permutable, but rather six univariate data sets.

(a) For rotation and static 2d measurements, plot back-to-back histograms (bihist.m) as well as boxplots. (b) For static 7d measurements graph by pie chart (pie) the proportion of EBs with circularity smaller than 0.75.