Cell Bio - Laboratory Exercises Library Lab Assignment .. Use www.PubMed.gov Part 1. Searching Techniques The purpose of this exercise is to familiarize you with searching scientific literature to gain additional information about particular topics. Formerly, this work was done exclusively in a library utilizing multi-volume, bound resources such as The Science Citation Index, Biological Abstracts, etc. Thankfully, it is much more convenient to conduct these searches at a computer terminal using the internet. Your assignment is to identify three key words associated with a cell physiology topic of your interest (some examples are provided below), and use an internet library resource to locate ten journal articles that address this topic from the last 5 years. You must then construct a bibliography in which these ten literature references are listed in alphabetical order in a standard journal format. You may use any format of your choice, as long as it includes the title of the article. The chosen format must be a standard format found in the reference section of a journal article that is typically in use, and it must be consistent for each listing. One example of an acceptable format is: Gardner, P.L., G.N.K. Mbuy, M.T. Knabb. (1994) Effects of the angiotensin II antagonist losartan on herpes simplex virus-type 2 infection of cultured vero and cardiac neonatal myocytes. Life Sci. 55(4): 283-289. You must then choose one of these articles, download the abstract from the internet and print a hard copy. You may use any internet resource of your choice. PubMed is a particularly useful and convenient search engine. This tax-payer-funded resource is maintained by the National Library of Medicine, part of the National Institutes of Health. For simplicity, you may find the PubMed site address through a Google search using "PubMed" as the search topic. Important Note: All references cited must be peer reviewed from the scientific literature. Is is not acceptable to use web references or sites such as “Wikipedia”, or non-scientific references such as “Time Magazine”. If in doubt, the reference such be similar to what appears at the end of a standard printed scientific research article, and the information should be sufficient so that the reader can physically look up the article in a reference library. Part 2. Dissecting a Research Paper . Research papers contain a lot of jargon, are often written for specialists, and the information is typically very densely packed. It is essential that students gain experience in reading and deciphering the scientific literature. For this assignment you are required to choose one scientific journal article relevant to cell physiology from your bibliography above, obtain a compete hard copy of the article (you will probably have to get it from the library), and read it carefully (you may have to read it several times to really understand it). You should then print the entire abstract, and provide a sentence by sentence translation of the complete abstract in your own words. Take each sentence and explain simply what it means within the context of the whole paper. You may need to craft two or three sentences to explain just one sentence in the abstract. Another way to think of this is that you are required to provide a “translation” of the scientific language in the abstract into regular plain English. Make sure that you choose a research article NOT a Review Article. Checklist for this assignment: What should be submitted? Part 1. Searching Techniques To turn in: ? Three key words relevant to a cell physiology topic. ? Alphabetical list of 10 references. Standard format of your choice; must be consistent and contain the title of the article. Note: Simple copy/pasting of references from PubMed is not acceptable. You may use EndNote, OneNote, or similar software for bibliography management, or else reconfigure it into proper reference format using Word. Do not use web references… ? Hard copy of one abstract. (You may copy/paste this into your Word document.) Part 2. Dissecting a Research Paper Choose 1 article from your list of 10 reference. Download and print the entire article. Read and re-read the article until you understand it. To turn in: ? The abstract of the article. (You may copy/paste this into your Word document.) ? The formatted reference citation for the article. ? Your "in-plain-English" interpretation of the abstract. Make sure this is double-spaced. Your summary should not exceed one page.Sample Key Words Design your own 3 key words or choose 3 words from the list below. 1) microorganisms, extreme environments, enzymes 2) tumor virus, oncogene, growth factor 3) bacteria, hydrocarbon, degradation 4) insects, cannibalism, chemical secretions 5) cholesterol, LDL, atherosclerosis 6) mitochondria, DNA, myopathy 7) virus, gene therapy, Alzheimer's 8) RNA, viroid, plant 9) membrane, freeze fracture, electronmicroscopy 10) prion, neuron, mad cow 11) sodium channel, receptor, purification 12) lysosome, formation, mannose-6-phosphate 13) pig, heart, xenotransplantation 14) inositol triphosphate, calcium, signal transduction 15) cyclic AMP, adenylate cyclase, G protein 16) programmed cell death, apoptosis, aging 17) adult cell, sheep, cloning 18) chaperones, protein, assembly 19) taxol, tubulin, mitosis 20) extracellular matrix, integrins, adhesion 21) Aplysia, memory, serotonin 22) nucleopores, protein, translocation 23) free radicals, antioxidants, aging 24) nitric oxide, smooth muscle, vasodilation 25) T cell, gp120, AIDS 26) cocaine, receptor, desensitization 27) dopamine, receptor, schizophrenia 28) mitochondria, DNA, evolution 29) chloride channel, cystic fibrosis, gene therapy 30) DNA, fingerprinting, forensics Some journals relevant to Cell Biology that are available at WCU You may use other journals, as long as they are peer-reviewed. See the WCU Library site for a current listing. Agricultural and Biological Chemistry American Journal of Physiology Annual Review of Biochemistry Annual review of cell biology Annual Review of Physiology Annual Review of Plant Physiology and Plant Molecular Biology Archives of Biochemistry and Biophysics Biochemical Journal Biochemical Pharmacology Biochimica et Biophysica Acta Biophysical Journal Cell Cellular and Molecular Biology Comparative Biochemistry and Physiology Cytologia Experimental Cell Research FASEB Journal Journal of Biological Chemistry Journal of Cell biology Journal of Cellular Physiology Journal of Histochemistry and Cytochemistry Journal of Lipid Research Journal of Molecular Biology Journal of Molecular Evolution Journal of Neurochemistry Journal of Ultrastructure and Molecular Structure Research Proceedings of the National Academy of Sciences Nature Photochemistry and Photobiology Plant and cell Physiology Plant Physiology Progress in Biophysics and Molecular Biology Science